Analysis of tRNA derived fragments (tRFs) in Triops cancriformis development

Material type: 文書・図像類

Author: 広瀨, 友香

Publisher: 慶應義塾大学湘南藤沢学会

Publication date: 2013

Material Format: Digital

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Note (General)：: type:textMicroRNAs (miRNAs) are 18-24 nucleotide (nt) non-coding RNAs that are deeply involved in development, especially in morphogenesis. However, t...

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Material Type: 文書・図像類
Title: Analysis of tRNA derived fragments (tRFs) in Triops cancriformis development
Author/Editor: 広瀨, 友香
Author Heading: 広瀨, 友香
Publication, Distribution, etc.: 慶應義塾大学湘南藤沢学会
Publication Date: 2013
Publication Date (W3CDTF): 2013
Periodical title: 生命と情報
No. or year of volume/issue: 20
Volume: 20
Pages: 48-54
Text Language Code: eng
Subject Heading: tRNA-derived fragment,small RNA,development,Triops cancriformis,deep sequencing
Target Audience: 一般
Note (General): type:text
MicroRNAs (miRNAs) are 18-24 nucleotide (nt) non-coding RNAs that are deeply involved in development, especially in morphogenesis. However, the detailed biological information about 25-45 nt long small RNAs remains unclear. Here, I aimed to perform a comprehensive analysis of 25-45 nt-long small RNAs in Triops cancriformis (Tadpole shrimp), whose morphology dramatically changes during development. I especially focused on stage-specific mitochondrial and genomic tRNA-derived fragments (tRFs). To detect small RNAs, deep sequencing of small RNA libraries in each six developmental stage (egg, 1st-4tn instar larvae, and adult) of T. cancriformis was performed. In order to analyze tRFs, I obtained mitochondrial DNA sequence from NCBI and genomic DNA sequences by deep sequencing, and predicted mitochondrial and genomic tRNA genes by tRNAscan-SE. By the comparative sequence analysis between tRNA genes and small RNAs, it was found that large amount of small RNAs were derived from mitochondrial and genomic tRNA genes. Interestingly, tRPs were derived from the particular positions of its tRNAs. For example, approximately 25-30 nt-long tRFs were produced from the almost similar positions around the 55 half regions of mitochondrial and genomic tRNALys(CUU) at every six stage. Moreover, expression pattern analysis suggested that the expression of many mitochondrial and genomic tRFs was altered in six developmental stages. Northern blot analysis confirmed that two genomic tRFs were actually expressed in T. cancriformis. In particular, several tRFs in different length were produced from genomic tRNALys(CUU) depending on the developmental stages. These results suggest that tRFs are not random degradation products, but may have important role(s) in T. cancriformis development.
慶應義塾大学湘南藤沢キャンパス先端生命科学研究会 2013年度学生論文集修士論文ダイジェスト
identifier:SFC-RM2013-005
identifier:請求記号: 090@KE1@179-20-1 請求記号: 090@KE1@179-20-2
Access Restrictions: 限定公開
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ