妊娠恒常性維持機構における羊膜マトリクス細胞蛋白の役割

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Author: 峰岸, 一宏ほか

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Publication date: 2014

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Note (General)：: type:text(1) 各妊娠時期での羊膜マトリクス細胞蛋白の発現変化 : SPARC mRNA発現は, 妊娠後期に40%減少し, TSP1は3倍に増加した。(2) 羊膜プロスタグランジン合成酵素の発現変化 : COX2 mRNAのみが妊娠後期に2倍に増加した。(3) 流動細胞計測法を用いた羊膜上...

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科研費研究者番号 : 30276331

科研費研究者番号 : 10212937

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Material Type: 文書・図像類
Title: 妊娠恒常性維持機構における羊膜マトリクス細胞蛋白の役割
Author/Editor: 峰岸, 一宏
石本, 人士
Author Heading: 峰岸, 一宏
石本, 人士
Publication Date: 2014
Publication Date (W3CDTF): 2014
Alternative Title: ニンシンコウジョウセイイジキコウニオケルヨウマクマトリクスサイボウタンパクノヤクワリ
Ninshin kojosei iji kiko ni okeru yomaku matorikusu saibo tanpaku no yakuwari
The role of matrix cellular proteins in amniotic membrane
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
eng
Subject Heading: 羊膜上皮細胞,マトリクス細胞蛋白
Target Audience: 一般
Note (General): type:text
(1) 各妊娠時期での羊膜マトリクス細胞蛋白の発現変化 : SPARC mRNA発現は, 妊娠後期に40%減少し, TSP1は3倍に増加した。(2) 羊膜プロスタグランジン合成酵素の発現変化 : COX2 mRNAのみが妊娠後期に2倍に増加した。(3) 流動細胞計測法を用いた羊膜上皮細胞分離法の確立 : 当初の上皮細胞増殖が不安定で, 実験計画の変更を余儀なくされたため, 新たな分離法を確立した。(4) 羊膜上皮細胞における網羅的遺伝子発現解析 : 初代培養で増殖因子のmidkineやFGF2発現が低く, 免疫抑制因子HLA-G発現は高かった。継代後midkine, FGF2の発現は急速に増加し, HLA-G 発現は減少した。 (1) Expression changes of matrix cellular proteins of the amniotic membrane in mid and late gestation : the expression of SPARC mRNA was reduced by 40% at the late gestation. Expression of TSP1 was increased three-fold in late gestation. (2) Expression changes of prostaglandin synthesis enzyme : only COX2 mRNA expression was increased twice at the late gestation. (3) Establishment of amniotic epithelial cell separation method using flow cytometry : because there was instability in the cell growth of the original amniotic epithelial cells, it was forced to change the experimental plan. A new separation method was established. (4) Comprehensive gene expression analysis in epithelial cells obtained by flow cytometry : in primary epithelial cells, the expression of midkine and FGF2 involved in the proliferation was low, the expression of HLA-G related to immunosuppression was higher. After passage, the expression of midkine and FGF2 was increased rapidly, HLA-G expression was decreased.
研究種目 : 基盤研究(C) 研究期間 : 2011～2014 課題番号 : 23592414 研究分野 : 産婦人科
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 30276331
科研費研究者番号 : 10212937
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ