癌抑制型microRNAを用いた子宮体癌治療 : 抗癌剤感受性増強とExocure

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Author: 阪埜, 浩司

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Publication date: 2015

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Note (General)：: type:text【目的】子宮体癌においてエピジェネティックに発現抑制される癌抑制型microRNAを同定し, microRNAの効果および抗癌剤との併用による抗腫瘍効果を検討する。【結果】4種の子宮体癌細胞株に対し脱メチル化処理を行った結果, 全ての細胞株に共通して発現上昇するmicroRNAと...

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Material Type: 文書・図像類
Title: 癌抑制型microRNAを用いた子宮体癌治療 : 抗癌剤感受性増強とExocure
Author/Editor: 阪埜, 浩司
Author Heading: 阪埜, 浩司
Publication Date: 2015
Publication Date (W3CDTF): 2015
Alternative Title: ガンヨクセイガタ microRNA オモチイタシキュウタイガンチリョウ : コウガンザイカンジュセイゾウキョウト Exocure
Gan yokuseigata microRNA o mochiita shikyutaigan chiryo : koganzai kanjusei zokyo to Exocure
Endometrial cancer treatment by tumor suppressor microRNA
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
eng
Subject Heading: 子宮体癌,エピジェネティクス,miR-34b,メチル化
Target Audience: 一般
Note (General): type:text
【目的】子宮体癌においてエピジェネティックに発現抑制される癌抑制型microRNAを同定し, microRNAの効果および抗癌剤との併用による抗腫瘍効果を検討する。【結果】4種の子宮体癌細胞株に対し脱メチル化処理を行った結果, 全ての細胞株に共通して発現上昇するmicroRNAとしてmiR-34bを同定した。また, 子宮体癌細胞株にmiR-34bを導入することにより, コロニー形成能や細胞遊走能の低下が認められた(p＜0.05)。さらに, HEC-1Bをヌードマウスの皮下に移植し各薬剤を投与した。その結果, パクリタキセル＋miR-34b群は他の群に比し有意に腫瘍径の縮小が認められた(p＜0.05)。 [Objective] microRNAs have key roles in the onset, development and drug resistance of cancer. We identified miR-34b as a tumor suppressor-type microRNA with expression that is epigenetically suppressed in endometrial cancer. In this study, the antitumor effect of combined miR-34b and antitumor drugs on endometrial cancer was examined. [Results] There was no change in cell viability after administration of miR-34b following application of cisplatin or adriamycin to HEC-108, HEC-1B and KLE cells. However, after treatment with paclitaxel, cell viability after administration of miR-34b decreased in all three cell lines. Tumor development occurred 14 days after transplantation of HEC-1B cells in nude mice. Drugs were administered on that day and thereafter. The tumor diameter significantly decreased in mice treated with paclitaxel + miR-34b compared with the results for other treatments (p＜0.05).
研究種目 : 基盤研究(C)(一般) 研究期間 : 2013～2015 課題番号 : 25462608 研究分野 : 婦人科腫瘍学
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 70265875
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ