心筋分化における長鎖non-coding RNAの制御機構の解明

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Author: 西山, 崇比古

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Publication date: 2015

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Note (General)：: type:text本研究では, non coding RNAでもまだ機能が十分に解明されていないlinc RNAに着目した。心臓発生と心筋分化誘導下において必要なlinc RNAを同定するためにマウス胎児, 出生後, 成獣からRNAを抽出した。また, ES細胞から心筋分化誘導し作成した心筋細胞と未分...

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科研費研究者番号 : 20464844

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Material Type: 文書・図像類
Title: 心筋分化における長鎖non-coding RNAの制御機構の解明
Author/Editor: 西山, 崇比古
Author Heading: 西山, 崇比古
Publication Date: 2015
Publication Date (W3CDTF): 2015
Alternative Title: シンキンブンカニオケルチョウサ non-coding RNA ノセイギョキコウノカイメイ
Shinkin bunka ni okeru chosa non-coding RNA no seigyo kiko no kaimei
Long non coding RNAs regulate the cardiac lineage during embryonic stem cell differentiation
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
eng
Subject Heading: long non coding RNA
Target Audience: 一般
Note (General): type:text
本研究では, non coding RNAでもまだ機能が十分に解明されていないlinc RNAに着目した。心臓発生と心筋分化誘導下において必要なlinc RNAを同定するためにマウス胎児, 出生後, 成獣からRNAを抽出した。また, ES細胞から心筋分化誘導し作成した心筋細胞と未分化状態のES細胞からRNAを抽出した。これらの, サンプルをnon coding RNA arrayを行い, 発生段階に発現量の変化を認めた心臓発生に必要と考えられるlinc RNAを特定することができた。また, RT-PCRにより同lincRNAの発現量がarrayと一致することを確認した。 Long non coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides, but they do not code proteins. LncRNAs constitute a heterogenic class of RNAs that includes intergenic RNAs, antisense transcripts, and enhancer RNAs. In recent, several studies have been reported that lncRNAs required for cardiac development and disease. It is obvious that many lncRNAs are expressed in cardiac development and are necessary for cardiovascular lineage. However, it has not been investigated in detail how lncRNAs are regulated in cardiac development and heart failure.Using embryonic stem cell (ESC) differentiation strategies, lncRNAs which restricted to cardiac cell were analyzed. In this study, to establish a map of lncRNAs expression and potential target gene networks during ESC differentiation. We identified the potential target genes of these lncRNAs through a bioinformatic correlation network that clusters lncRNAs into specific functional pathway.
研究種目 : 若手研究(B) 研究期間 : 2013～2015 課題番号 : 25860621 研究分野 : 循環器内科
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 20464844
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ