精子形成減数分裂期染色体におけるSINE(B1F)RNAの機能解析

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Author: 野瀬, 俊明ほか

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Publication date: 2015

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Note (General)：: type:textES細胞からのin vitro生殖細胞分化を解析する中で, 誘導開始時に発現上昇する一つの非コードRNA(R53)を同定した。R53は約80塩基の最終RNA産物を生じ, その配列にはSINE-B1モチーフを含み, 生体内発現は精巣の減数分裂M期相同染色体に局在するという極めて特異な...

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科研費研究者番号 : 70183902

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Material Type: 文書・図像類
Title: 精子形成減数分裂期染色体におけるSINE(B1F)RNAの機能解析
Author/Editor: 野瀬, 俊明
中島, 龍介
Author Heading: 野瀬, 俊明
中島, 龍介
Publication Date: 2015
Publication Date (W3CDTF): 2015
Alternative Title: セイシケイセイゲンスウブンレツキセンショクタイニオケル SINE (B1F) RNA ノキノウカイセキ
Seishi keisei gensu bunretsuki senshokutai ni okeru SINE (B1F) RNA no kino kaiseki
A small non-coding RNA containing SINE/B1 motif functions in meiotic metaphase germ cells during spermatogenesis
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
eng
Subject Heading: 非コードRNA,精子形成,減数分裂
Target Audience: 一般
Note (General): type:text
ES細胞からのin vitro生殖細胞分化を解析する中で, 誘導開始時に発現上昇する一つの非コードRNA(R53)を同定した。R53は約80塩基の最終RNA産物を生じ, その配列にはSINE-B1モチーフを含み, 生体内発現は精巣の減数分裂M期相同染色体に局在するという極めて特異な特性を示した。その生理機能を調べるため, 精巣器官培養に対してshRNAレンチベクターを用いたKnock-Down実験を行った結果, 対照区には見られない顕著な精子細胞分化の阻害効果が確認された。この研究成果はSINE-B1 RNA転写物の非コードRNAが精子形成分化の制御に関わる機能を持つことを示す初めての実証例となった。 Transcripts derived from a small subset of SINEs have been known to be expressed during spermatogenesis, whereas their functions remain unknown. We have identified a non-coding RNA, R53, which starts to express shortly after germ cell induction from ES cells in vitro. The mature product of R53 is ca. 80 base-RNA containing a SINE/B1 motif. Surprisingly, R53 RNA shows an unique accumulation on the pairing chromosomes in meiotic cells at the MI/II-meiotic stage. To know the function of R53 RNA, we examined knock-down effect on spermatogenesis by micro-injection of lenti-virus expressing R53 shRNA into testis tubules in an organ-culture. When testes of Acrosin-GFP tg mice were used as recipients and cultured for 2-3 weeks, differentiation of GFP-positive spermatids was significantly inhibited by R53 knock-down, suggesting that a specific SINE/B1-derived small RNA plays a critical role for regulating meiotic cell division and/or post-meiotic gene expression.
研究種目 : 挑戦的萌芽研究研究期間 : 2014～2015 課題番号 : 26650129 研究分野 : 発生生物学
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 70183902
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ