iPS細胞および脱細胞化・再細胞化技術を用いた子宮再構築

Material type: 文書・図像類

Author: 宮崎, 薫

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Publication date: 2015

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Note (General)：: type:textラットiPS細胞を購入し, レンチウィルスベクターにてGFP遺伝子およびluciferase遺伝子を導入した。FACSにてGFP陽性細胞を分取し, GFP陽性ラットiPS細胞のクローニングに成功した。GFP陽性ラットiPS細胞を免疫不全マウスに皮下移植し, in vivo imag...

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科研費研究者番号 : 90445370

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Material Type: 文書・図像類
Title: iPS細胞および脱細胞化・再細胞化技術を用いた子宮再構築
Author/Editor: 宮崎, 薫
Author Heading: 宮崎, 薫
Publication Date: 2015
Publication Date (W3CDTF): 2015
Alternative Title: iPS サイボウオヨビダツサイボウカ・サイサイボウカギジュツオモチイタシキュウサイコウチク
iPS saibo oyobi datsusaiboka saisaiboka gijutsu o mochiita shikyu saikochiku
Reconstruction of the uterus through differentiation of induced pluripotent stem cells within decellularized uterine matrix
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
eng
Subject Heading: 生殖医学,組織再構築,脱細胞化,再細胞化
Target Audience: 一般
Note (General): type:text
ラットiPS細胞を購入し, レンチウィルスベクターにてGFP遺伝子およびluciferase遺伝子を導入した。FACSにてGFP陽性細胞を分取し, GFP陽性ラットiPS細胞のクローニングに成功した。GFP陽性ラットiPS細胞を免疫不全マウスに皮下移植し, in vivo imaging systemを用いた細胞発光の追跡が可能であることを確認した。さらに, 6週間後の奇形腫形成を確認した。ラットiPS細胞を, 各分化段階に応じて培養液中の各種増殖因子の組成を変えながら培養した。リアルタイムPCRにて, ラットiPS細胞が中胚葉を経てミュラー管系統に分化していることが示唆された。 Rat iPS cells (riPSCs) were purchased and propagated. GFP gene and the luciferase gene were introduced in riPSCs with the lentiviral vector. GFP-positive riPSCs were cloned by single cell sorting of GFP-positive cells using FACS. GFP-positive riPSCs were transplanted into immunodeficient mice subcutaneously, and the cellular luminescence, which was generated by degradation of luciferin catalyzed by luciferase, and reflects the cell growth, was non-invasively tracked using in vivo imaging system. Teratoma formation was confirmed 6weeks after the transplantation. RiPSCs were cultured in a medium supplemented with several growth factors for differentiation. Quantitative real-time PCR was performed to confirm the expression of the gene markers, and the results suggested that riPSCs underwent differentiation into Mullerian lineage via intermediate mesoderm.
研究種目 : 若手研究(B) 研究期間 : 2014～2015 課題番号 : 26861340 研究分野 : 生殖医学
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 90445370
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ