椎間板髄核の組織恒常性維持機構の解析

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Author: 藤田, 順之ほか

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Publication date: 2017

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Note (General)：: type:text我々はU-CH1-N細胞株を用いて, 髄核細胞との類似性を検証した結果, U-CH1-Nは髄核の分子マーカーであるBrachyury (T), CD24, Cytokeratin19の発現が高く, 更には軟骨基質であるAggrecanやType II collagenの発現も高く,...

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Material Type: 文書・図像類
Title: 椎間板髄核の組織恒常性維持機構の解析
Author/Editor: 藤田, 順之
松本, 守雄
Author Heading: 藤田, 順之
松本, 守雄
Publication Date: 2017
Publication Date (W3CDTF): 2017
Alternative Title: ツイカンバンズイカクノソシキコウジョウセイイジキコウノカイセキ
Tsuikanban zuikaku no soshiki kōjōsei iji kikō no kaiseki
Tissue homeostasis of intervertebral disc
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
eng
Subject Heading: 椎間板,髄核,繊維輪,酸化ストレス,細胞株
Target Audience: 一般
Note (General): type:text
我々はU-CH1-N細胞株を用いて, 髄核細胞との類似性を検証した結果, U-CH1-Nは髄核の分子マーカーであるBrachyury (T), CD24, Cytokeratin19の発現が高く, 更には軟骨基質であるAggrecanやType II collagenの発現も高く, 長期に培養したところ, アルシアンブルー陽性であり, 脊索由来の髄核細胞と分子生物学的に非常に類似した特徴を持つ細胞株であることが判明した。本細胞株に対してカチオン性脂質を用いた遺伝子導入でも高い導入効率を示し, 分子生物学的手法を用いた研究においても非常に有用であった。 We show that a chordoma-derived cell line U-CH1-N cells highly express the nucleus pulposus (NP) marker genes, including T, KRT19, and CD24. These observations were further confirmed by immunocytochemistry and flow cytometry. Reporter analyses showed that transcriptional activity of T was enhanced in U-CH1-N cells. Chondrogenic capacity of U-CH1-N cells was verified by evaluating the expression of ECM genes and Alcian blue staining. Of note, we found that proliferation and synthesis of chondrogenic ECM proteins were largely dependent on T in U-CH1-N cells. In accordance, knockdown of the T transcripts suppressed the expression of PCNA, a gene essential for DNA replication, and SOX5 and SOX6, the master regulators of chondrogenesis. On the other hand, the CD24-silenced cells showed no reduction in the mRNA expression level of the chondrogenic ECM genes. These results suggest that U-CH1-N shares important biological properties with notochordal NP cells.
研究種目 : 基盤研究(C)(一般) 研究期間 : 2015～2017 課題番号 : 15K10420 研究分野 : 運動器
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 30348685
科研費研究者番号 : 40209656
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ