アクチン動態の制御に基づくがん幹細胞の分化転換誘導治療法の開発

Material type: 文書・図像類

Author: 信末, 博行

Publisher: -

Publication date: 2017

Material Format: Digital

Capacity, size, etc.: -

NDC: -

View All

Notes on use

Note (General)：: type:text骨肉腫幹(OSi)細胞において, 間葉系幹細胞の性質を有するAO細胞は抗癌剤(アドリアマイシン及びシスプラチン)に対して高い抵抗性を示した。抗癌剤耐性のAO細胞にROCK阻害薬のFasudilを単剤投与すると, アクチン細胞骨格の動態変化を介して転写調節因子のMKL1の核移行及び転...

Related materials as well as pre- and post-revision versions

科研費研究者番号 : 90525685

Search by Bookstore

Holdings of Libraries in Japan

This page shows libraries in Japan other than the National Diet Library that hold the material.

List of Cooperating Institutions and Databases

Please contact your local library for information on how to use materials or whether it is possible to request materials from the holding libraries.

other

Keio Associated Repository of Academic resources
Digital
You can check the holdings of institutions and databases with which Institutional Repositories DataBase(IRDB)(Institutional Repository) is linked at the site of Institutional Repositories DataBase(IRDB)(Institutional Repository).
Keio Associated Repository of Academic resources

Search by Bookstore

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 文書・図像類
Title: アクチン動態の制御に基づくがん幹細胞の分化転換誘導治療法の開発
Author/Editor: 信末, 博行
Author Heading: 信末, 博行
Publication Date: 2017
Publication Date (W3CDTF): 2017
Alternative Title: アクチンドウタイノセイギョニモトズクガンカンサイボウノブンカテンカンユウドウチリョウホウノカイハツ
Akuchin dōtai no seigyo ni motozuku gan kansaibō no bunka tenkan yūdō chiryōhō no kaihatsu
Therapeutic strategies for cancer stem cells by regulating cellular differentiation based on actin dynamics
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
eng
Subject Heading: 分化制御,アクチン細胞骨格,骨肉腫
Target Audience: 一般
Note (General): type:text
骨肉腫幹(OSi)細胞において, 間葉系幹細胞の性質を有するAO細胞は抗癌剤(アドリアマイシン及びシスプラチン)に対して高い抵抗性を示した。抗癌剤耐性のAO細胞にROCK阻害薬のFasudilを単剤投与すると, アクチン細胞骨格の動態変化を介して転写調節因子のMKL1の核移行及び転写が抑制され, 脂肪細胞への終末分化が誘導された。またFasudilはin vitro及びin vivoでのAO細胞の腫瘍形成性を抑制した。以上の結果から, 抗癌剤耐性のAO細胞のアクチン細胞骨格の動態を制御することで, 脂肪分化転換を誘導し腫瘍抑制できることが明らかとなった。 Osteosarcoma-initiating (OSi) cells are composed of two distinct types : AO cells, which have characteristics similar to mesenchymal stem cells, and AX cells, which have those similar to osteo-chondro progenitor cell and highly tumorigenic. We found that AO cells are highly resistant to chemotherapeutic agents, such as adriamycin and cisplatin. Treatment with the ROCK inhibitor fasudil alone induced remodeling of the actin cytoskeleton and inactivation of the transcriptional coactivator MKL1, resulting in the terminal adipocyte differentiation of chemoresistant stem-like AO cells. Furthermore, fasudil significantly suppressed in vitro growth and in vivo tumorigenesis of AO cells. Our findings suggest that induction of trans-differentiation by regulating actin cytoskeleton dynamics is a potential approach for chemoresistant stem-like OS cells.
研究種目 : 若手研究(B) 研究期間 : 2016～2017 課題番号 : 16K18426 研究分野 : 腫瘍生物学
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 90525685
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ