尿路上皮癌微小環境における新規上皮間葉転換制御機構の解明

Material type: 文書・図像類

Author: 大門, 達明

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Publication date: 2019

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Note (General)：: type:textヒト尿路上皮癌細胞株におけるEPB4.1L5の発現と各種転写因子等、EMT関連タンパクの発現の検討として浸潤能が異なる各種ヒト尿路上皮癌の細胞株T24、UMUC-3、5637を用い EPB4.1L5の発現を検討した。タンパク発現量はUMUC-3>T24>5637の順にであり、浸潤能...

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科研費研究者番号 : 40573275

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Material Type: 文書・図像類
Title: 尿路上皮癌微小環境における新規上皮間葉転換制御機構の解明
Author/Editor: 大門, 達明
Author Heading: 大門, 達明
Publication Date: 2019
Publication Date (W3CDTF): 2019
Alternative Title: ニョウロジョウヒガンビショウカンキョウニオケルシンキジョウヒカンヨウテンカンセイギョキコウノカイメイ
Nyōro jōhigan bishō kankyō ni okeru shinki jōhi kan'yō tenkan seigyo kikō no kaimei
Epithelial to mesenchymal transition in urothelial cancer
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
eng
Subject Heading: 尿路上皮癌,上皮間葉転換
Target Audience: 一般
Note (General): type:text
ヒト尿路上皮癌細胞株におけるEPB4.1L5の発現と各種転写因子等、EMT関連タンパクの発現の検討として浸潤能が異なる各種ヒト尿路上皮癌の細胞株T24、UMUC-3、5637を用い EPB4.1L5の発現を検討した。タンパク発現量はUMUC-3>T24>5637の順にであり、浸潤能の傾向と一致した。低酸素状態ではEMT関連転写因子の発現は高くなるものの、EPB4.1L5の発現はむしろ低下する結果となった。また、HIF-1α阻害剤下においても、発現に変化は認めなかった。シスプラチン耐性株での検討を行ったところ、EMT関連転写因子の発現は高くなるものの、EPB4.1L5の発現に変化は認めなかった。 We examined the protein expression of EPB4.1L5 and EMT related transcriptional factors by western blotting in human urothelial cell lines, UMUC-3, T24 and 5637. The protein expression accorded with a tendency of the invasion ability in order of UMUC-3>T24>5637. In the hypoxic state, although the expression of EMT-related transcription factor became higher, the protein expression of EPB4.1L5 became lower unexpectedly. In addition, there was no significant difference in the protein expression of EPB4.1L5 with or without the HIF-1α inhibitor. Although the expression of EMT-related transcription factors like Snail were higher in the cisplatin-resistant T24 cell line compared with the original T24 cell line, there was no difference between the expression of the protein of the two cell lines.
研究種目 : 若手研究研究期間 : 2018～2019 課題番号 : 18K16710 研究分野 : 泌尿器科学
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 40573275
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ