無痛型神経成長因子を活用した痛み特異的シグナリングの同定と神経再生への応用

Material type: 文書・図像類

Author: 加藤, 純悟

Publisher: -

Publication date: 2022

Material Format: Digital

Capacity, size, etc.: -

NDC: -

View All

Notes on use

Note (General)：: type:text我々はより安全な鎮痛を目指して、神経成長因子(NGF)による痛み特異的シグナリングの特定を進めてきた。NGF-p75結合を選択的に阻害する抗NGF抗体NGF30をもとに精製したNGF30-F(ab’)2は、PC 12細胞においてNGF受容体TrkAのinternalizationを...

Related materials as well as pre- and post-revision versions

科研費研究者番号 : 40465018

Search by Bookstore

Holdings of Libraries in Japan

This page shows libraries in Japan other than the National Diet Library that hold the material.

List of Cooperating Institutions and Databases

Please contact your local library for information on how to use materials or whether it is possible to request materials from the holding libraries.

other

Keio Associated Repository of Academic resources
Digital
You can check the holdings of institutions and databases with which Institutional Repositories DataBase(IRDB)(Institutional Repository) is linked at the site of Institutional Repositories DataBase(IRDB)(Institutional Repository).
Keio Associated Repository of Academic resources

Search by Bookstore

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 文書・図像類
Title: 無痛型神経成長因子を活用した痛み特異的シグナリングの同定と神経再生への応用
Author/Editor: 加藤, 純悟
Author Heading: 加藤, 純悟
Publication Date: 2022
Publication Date (W3CDTF): 2022
Alternative Title: ムツウガタシンケイセイチョウインシオカツヨウシタイタミトクイテキシグナリングノドウテイトシンケイサイセイエノオウヨウ
Mutsūgata shinkei seichō inshi o katsuyōshita itami tokuiteki shigunaringu no dōtei to shinkei saisei eno ōyō
Identification of pain-specific nerve growth factor (NGF)-mediated signalings using painless NGF and application to painless nerve regeneration therapy
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
eng
Subject Heading: 痛み,慢性痛,神経成長因子,神経再生,p75
Target Audience: 一般
Note (General): type:text
我々はより安全な鎮痛を目指して、神経成長因子(NGF)による痛み特異的シグナリングの特定を進めてきた。NGF-p75結合を選択的に阻害する抗NGF抗体NGF30をもとに精製したNGF30-F(ab’)2は、PC 12細胞においてNGF受容体TrkAのinternalizationを促進することが示された。またマウス足掌へのNGF投与による疼痛誘発モデルではNGF30-F(ab’)2の投与が有意に機械的過敏性亢進を抑制し、投与部位での異常な交感神経発芽を抑制することも示された。以上より、NGF30-F(ab’)2が　有効な鎮痛効果を示すとともに痛みを伴わない神経再生への活路が見出された。 We have attempted to develop a novel analgesic by targeting pain-specific nerve growth factor-mediated signal pathways. We produced F(ab’)2 fragments from anti-NGF monoclonal antibody NGF30 that reportedly specifically inhibits NGF-p75 interactions. The NGF30- F(ab’)2 fragments facilitated the NGF-induced internalization of NGF cognate receptor TrkA in PC12 cells. In vivo model, in which NGF was injected into the plantar area of hindpaw in mice, revealed that the addition of F(ab’)2 fragments significantly attenuated the NGF-induced mechanical hypersensitivity induced by NGF injection. Furthermore, the addition of F(ab’)2 fragments also suppressed NGF-induced abnormal sympathetic innervation in the NGF-injected planter skin. These findings suggest that the F(ab’)2 fragment can be a promising candidate for analgesic and painless nerve regeneration therapies.
研究種目 : 若手研究研究期間 : 2020～2022 課題番号 : 20K17821 研究分野 : 麻酔学
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 40465018
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ