自由大気圏を長距離移動するバイオエアロゾルが遺伝子の水平伝播に及ぼす影響

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Author: 柿川, 真紀子ほか

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Publication date: 2015-06-10

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Note (General)：: 金沢大学環日本海域環境研究センター本研究では黄砂発生源地の一つであるタクラマカン砂漠の東端にある敦煌市と石川県上空において、エアロゾル（大気浮遊物質）を採集後、DNA抽出し、DNA濃度から微生物濃度を明らかにした。また、rRNA遺伝子や組み換え遺伝子の配列解析により微生物の多様性や同定を行なった。そ...

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Material Type: 文書・図像類
Title: 自由大気圏を長距離移動するバイオエアロゾルが遺伝子の水平伝播に及ぼす影響
Author/Editor: 柿川, 真紀子
Kakikawa, Makiko
Author Heading: 柿川, 真紀子
Kakikawa, Makiko
Publication Date: 2015-06-10
Publication Date (W3CDTF): 2015-06-10
Alternative Title: Molecular analysis of bioaerosols in the atmosphere over China and Japan
Periodical title: 平成26(2014)年度科学研究費補助金基盤研究(B) 研究成果報告書 = 2014 Fiscal Year Final Research Report
No. or year of volume/issue: 2012-04-01 – 2015-03-31
Volume: 2012-04-01 – 2015-03-31
Pages: 6p.-
Text Language Code: jpn
Subject Heading: 微生物
DNA配列
エアロゾル
Target Audience: 一般
Note (General): 金沢大学環日本海域環境研究センター
本研究では黄砂発生源地の一つであるタクラマカン砂漠の東端にある敦煌市と石川県上空において、エアロゾル（大気浮遊物質）を採集後、DNA抽出し、DNA濃度から微生物濃度を明らかにした。また、rRNA遺伝子や組み換え遺伝子の配列解析により微生物の多様性や同定を行なった。その結果、大気中のDNA濃度は黄砂飛来時には高くなること、地上付近になるほどDNA濃度は高くなること、生物の多様性は上空になるほど低くなる傾向が見出された。
In recent years, many epidemiological studies have shown that dust events are associated with an increase in risk of mortality and patients of cardiovascular and respiratory illness. However, there are few data about airborne bacteria. In this study, we investigated the airborne bacteria in the atmosphere over Taklimakan Desert in China, Asian dust source, and over Ishikawa prefecture in Japan. The airborne bacteria were collected on 0.45 um-pore size filter by vacuum pump, and their DNA were extracted from the filter and analyzed by their DNA sequence. The results suggested that DNA concentrations in the atmosphere over Ishikawa in Japan had been higher after dust storm in the deserts of China. Some airborne bacteria were identified by matching to the sequences of DNA database. Some results suggested that the diversity of airborne bacteria in the higher atmosphere was lower than that near the ground surface.
研究課題/領域番号:24403002, 研究期間(年度):2012-04-01 – 2015-03-31
DOI: 10.24517/00034476
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=10359713
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24403002/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24403002/24403002seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA