JNK結合分子による細胞接着斑と運動極性形成制御機構の解析

Material type: 文書・図像類

Author: 滝野, 隆久ほか

Publisher: 金沢大学がん研究所

Publication date: 2008-05-01

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Note (General)：: HT1080細胞のI型コラーゲンおよびファイブロネクチン(FN)上での細胞運動はMMP阻害剤BB94により抑制され、ECM分解の減少、細胞接着斑の過形成と運動極性の喪失が認められた。一方、MT1-MMPの過剰発現はECM分解、細胞運動と細胞接着斑の代謝回転を誘導し、阻害変異体の発現でその抑制が認めら...

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Material Type: 文書・図像類
Title: JNK結合分子による細胞接着斑と運動極性形成制御機構の解析
Author/Editor: 滝野, 隆久
Takino, Takahisa
Author Heading: 滝野, 隆久
Takino, Takahisa
Publication, Distribution, etc.: 金沢大学がん研究所
Publication Date: 2008-05-01
Publication Date (W3CDTF): 2008-05-01
Alternative Title: Analysis of a molecular mechanism by which JNK-binding proteins regulate turnover of focal adhesions and formAnalysis of a molecular mechanism by which JNK-binding proteins regulate turnover of focal adhesions and formation of cell polarity
Periodical title: 平成19(2007)年度科学研究費補助金基盤研究(C) 研究成果報告書 = 2007 Fiscal Year Final Research Report
No. or year of volume/issue: 2006-2007
Volume: 2006-2007
Pages: 4p.-
Text Language Code: jpn
Subject Heading: がん
浸潤
細胞運動
MAPK
JNK
ERK
MT1-MMP
細胞外マトリックス
Cancer
Invasion
Cell migration
ECM
Target Audience: 一般
Note (General): HT1080細胞のI型コラーゲンおよびファイブロネクチン(FN)上での細胞運動はMMP阻害剤BB94により抑制され、ECM分解の減少、細胞接着斑の過形成と運動極性の喪失が認められた。一方、MT1-MMPの過剰発現はECM分解、細胞運動と細胞接着斑の代謝回転を誘導し、阻害変異体の発現でその抑制が認められた。HT1080細胞のBB94処理は細胞接着により誘導されるERK活性化を抑制した。以上の結果からMT1-MMPは細胞接着により誘導されるERK活性化を調節することにより細胞接着斑の代謝回転と細胞運動を制御していることが示唆された(T. Takino et al., Experimental Cell Research,2006)。細胞運動時、MT1-MMPは細胞接着斑の集積と代謝回転が頻繁に起こる運動先端部の内側でECMを分解していた。細胞接着斑におけるMT1-MMPの機能に着目し、FAKの細胞接着斑標的ドメインをMT1-MMPとMT1-MMPの阻害変異体に付与し、細胞接着斑におけるMT1-MMPの役割を解析した。細胞接着斑に標的したMT1-MMPはFNへの接着後、迅速に細胞接着斑でFNを分解した。細胞接着斑に標的したMT1-MMPの阻害変異体はMT1-MMP発現細胞におけるFN分解と細胞運動、3次元ECMへの細胞浸潤を顕著に抑制した。以上の結果から、細胞接着斑におけるMT1-MMP機能の重要性と、その活性阻害ががん浸潤・転移抑制に効果的であることが示された(T. Takino et al., Cancer Research, 2007)。
Membrane-type 1 matrix metalloproteinase (MT1-MMP) has been implicated in tumor invasion and metastasis. We previously reported that extracellular matrix degradation by MT1-MMP regulates cell migration via modulating sustained integrin-mediated signals. In this study, MT1-MMP-expressing cells were plated onto fibronectin-coated plates and monitored for cell-matrix adhesion formation and fibronectin degradation. The fibronectin was degraded and removed in line with the cell migration track. The migrating cells showed a polarized morphology and were in contact with the edge of fibronectin through the leading edge in which cell-matrix adhesions are concentrated. Expression of MT1-MMP targeted to cell-matrix adhesions by fusing with the focal adhesion targeting (FAT) domain of focal adhesion kinase (FAK) promoted the initial fibronectin lysis at the cell periphery immediately after adhesion. These results suggest that fibronectin is degraded by MT1-MMP located at cell-matrix adhesions which are concentrated at the leading edge of the migrating cells. lb inhibit MT1-MMP at cell-matrix adhesion, the dominant negative form of MT1-MMP (MT1-Pex) was targeted to the cell-matrix adhesion by fusing with the FAT domain (MT1-Pex-FAT). MT1-Pex-FAT accumulated at cell-matrix adhesions and inhibited fibronectin degradation as well as FAK phosphorylation more effectively than parental MT1-Pex. MT1-Pex-FAT was also shown to suppress the invasion of tumor cells into 3-dimensional collagen gel more strongly than MT1-Pex. These results suggest that MT1-MMP-mediated extracellular matrix lysis at cell-matrix adhesions induces the establishment of cell polarity, which facilitates cell-matrix adhesion turnover and subsequent cell migration. This model highlights the role of MT1-MMP at the leading edge of migrating cells.
研究課題/領域番号:18590287, 研究期間(年度):2006–2007
出典：「JNK結合分子による細胞接着斑と運動極性形成制御機構の解析」研究成果報告書　課題番号18590287 (KAKEN：科学研究費助成事業データベース（国立情報学研究所）)　　　本文データは著者版報告書より作成
DOI: 10.24517/00034712
Source: https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=34725&item_no=1&attribute_id=26&file_no=1
https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=34725&item_no=1&attribute_id=26&file_no=1
Access Restrictions: 限定公開
Rights (production): CC BY-NC-ND
Related Material: https://kaken.nii.ac.jp/search/?qm=40322119
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590287/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18590287/185902872007kenkyu_seika_hokoku_gaiyo/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA