植物ゲノムにおける特定DNA配列の分布

• Material Type: 文書・図像類
• Title: 植物ゲノムにおける特定DNA配列の分布
• Author/Editor: 矢倉, 公隆
• Author Heading: 矢倉, 公隆
• Publication, Distribution, etc.: 金沢大学教育学部
• Publication Date: 1991-03-01
• Publication Date (W3CDTF): 1991-03-01
• Alternative Title: Distribution of Specific DNA Sequences in Plant Genomes
• Periodical title: 平成2年科学研究費補助金 一般研究(C) 研究成果報告書 = 1990 Fiscal Year Final Research Report
• No. or year of volume/issue: 1989-1990
• Volume: 1989-1990
• Pages: 16p.-
• Text Language Code: jpn
• Subject Heading: in situ ハイブリダイゼ-ション; Vicia属; ソラマメ; カラスノエンドウ; スズメノエンドウ; 反復配列; ARS; in situ hybridization; Genus Vicia; Vicia faba; Vicia angustufolia; Vicia hirsuta; repeated sequence
• Target Audience: 一般
• Note (General): 本研究は、植物の特定DNA配列がゲノム内でどのような分布をしているかを明らかにする事を目的とし、ソラマメ由来の高度反復配列であるBamHlファミリ-(250bp及び1500bp配列)あるいはタバコゲノムより単離した自己複製能をもった配列(ARS)をプロ-ブとしたサザン・ハイブリダイズ法及びin situハイブリダイズ法による解析を行い以下のような結果を得た。 1.ソラマメ(vicia faba)BamHl ファミリ-の250bpと1500bpの相同配列が、カラスノエンドウ(Vicia angustifolia)およびスズメノエンドウ(Vicia hirsuta)のソラマメ属の野生種にも分裂散型反復配列として存在することが明かとなり、さらに、250bp配列と1500bp配列は古くは互いに隣接した形でのみゲノム内に存在したいたものが、進化の過程で分離しながら別のファミリ-を形成するようになったということが示唆された。またカラスノエンドウ染色体における1500bp相同配列の分布に注目すると、ほとんどの場合、長腕あるいは短腕のテロルメもしくは動原体付近に見出され、長腕の中間の位置には存在しないか、存在していてもわずかであることが分かった。1500bp配列が全ての染色体の全長にわたって存在しているソラマメゲノムにおける分布様式とこの結果を対比させて考えると、この配列が進化と共に分散化が強まっていったことが示唆される。2.タバコ ARSと相同な配列はタバコ以外のいくつかの植物種にも存在することが分かった。その中で、ソラマメについてin situ ハイブリダイゼイションを行ったところ、明確な結果は未だ得られていないが、傾向として、銀粒子が各染色体に存在することを示唆するパタ-ンが多数得られた。今後、さらに比活性の高いプロ-ブを得るか、ARSと相同な配列をソラマメから単離しされをプロ-ブに用いることにより確かなデ-タを得る必要があろう。 The purpose of this research is to clarify distributional patterns of specific DNA sequences in plant genomes. The following results were obtained by Southern blot hybridization and in situ hybridization utilizing highly dispersed repeated sequences of Vicia faba BamHl families and a cloned autonomously replicating sequence (ARS) from tobacco nuclear genome as probes. 1. Sequences which have homology to 250bp and 1500bp BamHl family sequences of V. faba were also present as dispersed repeated sequences in genomes of two wild Vicia species, V. angustifolia and V. hirsuta. In addition, it was suggested that all ancester sequences of the 250bp and 1500bp BamHl families were arranged adjacently in the ancient time and some of them were split to generate other sequence families such as 250bp and 1500bp families in the process of evolution. In situ hybridization of the 1500bp sequence to 6pairs of subtelocentric chromosomes of V. angustifolia revealed that this sequence were localized in telomeric regions of short or long arms, or in the region near centromeres of each chromosome but not in the middle regions of long arms of any chromosomes. From the fact that the 1500bp sequence distributes diffusely throughout all the chromosomes of V. faba, it was also suggested that the extent of dispersion of this sequence was increased with the evolution of Vicia genome. 2. By Southern blot hybridization analysis it was found that sequences homologous to tobcco ARS were present in several other plant genomes. Although clear patterns were not obtained yet in situ hybrization using sequence homology to tobacco ARS as a probe showed that silver grains were seemed to be distributed every chromosomes of V. faba. In order to obtain fine results, it is necessary to use probe with higher specific activity or to clone ARS-homologows sequences from V. fava genome and use the probes.; The purpose of this research is to clarify distributional patterns of specific DNA sequences in plant genomes. The following results were obtained by Southern blot hybridization and in situ hybridization utilizing highly dispersed repeated sequences of Vicia faba BamHl families and a cloned autonomously replicating sequence (ARS) from tobacco nuclear genome as probes.1. Sequences which have homology to 250bp and 1500bp BamHl family sequences of V. faba were also present as dispersed repeated sequences in genomes of two wild Vicia species, V. angustifolia and V. hirsuta. In addition, it was suggested that all ancester sequences of the 250bp and 1500bp BamHl families were arranged adjacently in the ancient time and some of them were split to generate other sequence families such as 250bp and 1500bp families in the process of evolution. In situ hybridization of the 1500bp sequence to 6pairs of subtelocentric chromosomes of V. angustifolia revealed that this sequence were localized in telomeric regions of short or long arms, or in the region near centromeres of each chromosome but not in the middle regions of long arms of any chromosomes. From the fact that the 1500bp sequence distributes diffusely throughout all the chromosomes of V. faba, it was also suggested that the extent of dispersion of this sequence was increased with the evolution of Vicia genome.2. By Southern blot hybridization analysis it was found that sequences homologous to tobcco ARS were present in several other plant genomes. Although clear patterns were not obtained yet in situ hybrization using sequence homology to tobacco ARS as a probe showed that silver grains were seemed to be distributed every chromosomes of V. faba. In order to obtain fine results, it is necessary to use probe with higher specific activity or to clone ARS-homologows sequences from V. fava genome and use the probes.; 研究期間(年度):1989-1990, 研究課題/領域番号:01540562; 出典：「植物ゲノムにおける特定DNA配列の分布」研究成果報告書 課題番号01540562 (KAKEN：科学研究費助成事業データベース（国立情報学研究所）) 本文データは著者版報告書より作成
• Source: https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=34928&item_no=1&attribute_id=26&file_no=1
• Access Restrictions: 限定公開
• Rights (production): CC BY-NC-ND
• Related Material: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01540562/; https://kaken.nii.ac.jp/search/?qm=50166485; https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-01540562/015405621990kenkyu_seika_hokoku_gaiyo/
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA