骨制御分子Runx2による中枢制御機構の解明

Material type: 文書・図像類

Author: 宝田, 剛志ほか

Publisher: 金沢大学医薬保健研究域薬学系

Publication date: 2013-05-23

Material Format: Digital

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Note (General)：: 金沢大学医薬保健研究域薬学系我々は、骨芽細胞特異的表現型を特徴付けるRunt-related transcription factor 2(Runx2)分子が、中枢神経系にも機能的に発現する事実を報告した。本研究では、個体レベルでのRunx2の脳機能解析を実施する目的で、Cre/loxPシステムを利...

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Material Type: 文書・図像類
Title: 骨制御分子Runx2による中枢制御機構の解明
Author/Editor: 宝田, 剛志
Takarada, Takeshi
Author Heading: 宝田, 剛志
Takarada, Takeshi
Publication, Distribution, etc.: 金沢大学医薬保健研究域薬学系
Publication Date: 2013-05-23
Publication Date (W3CDTF): 2013-05-23
Alternative Title: Regulatory mechanisms of central nervous system by Runx2
Periodical title: 平成24(2012)年度科学研究費補助金基盤研究(C) 研究成果報告書 = 2012 Fiscal Year Final Research Report
No. or year of volume/issue: 2010-2012
Volume: 2010-2012
Pages: 4p.-
Text Language Code: jpn
Subject Heading: 神経細胞
アストロサイト
Runx2
コンディショナル欠損マウス
Target Audience: 一般
Note (General): 金沢大学医薬保健研究域薬学系
我々は、骨芽細胞特異的表現型を特徴付けるRunt-related transcription factor 2(Runx2)分子が、中枢神経系にも機能的に発現する事実を報告した。本研究では、個体レベルでのRunx2の脳機能解析を実施する目的で、Cre/loxPシステムを利用したRunx2 conditional欠損マウスの作製に取り組み、Runx2遺伝子のexon 4をloxP配列にて挟んだRunx2^flox/+のマウス作製に成功した。
We have previously shown the functional expression of glutamatergic and GABAergic signaling machineries in different osseous cells including osteoblasts. Runt-related factor 2 (Runx2) is the master regulator of osteoblastic differentiation with ability to accelerate differentiation of mesenchymal stem cells into osteoblasts, while we have also demonstrated the expression of mRNA and corresponding protein for Runx2. In this study, we for the first time generated mice carrying a conditional Runx2 allele with exon 4, which encodes the Runt domain, flanked by loxP sites. These mice were crossed with α1(I)-collagen-Cre or α1(II)-collagen-Cre transgenic mice to obtain osteoblast- or chondrocyte-specific Runx2 deficient mice, respectively. In newborn α1(II)-Cre;Runx2^flox/flox mice, mineralization impairment was restricted to skeletal areas undergoing endochondral ossification including long bones and vertebrae. In contrast, no apparent skeletal abnormalities were seen in mutant embryo, newborn, and 3- to 6-week old-mice in which Runx2 had been deleted with the α1(I)-collagen-Cre driver. The Runx2 floxed allele established here is undoubtedly useful for investigating the role of Runx2 in particular cells.
研究課題/領域番号:22500330, 研究期間(年度):2010-2012
出典：研究課題「骨制御分子Runx2による中枢制御機構の解明」課題番号22500330（KAKEN：科学研究費助成事業データベース（国立情報学研究所））（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-22500330/22500330seika/）を加工して作成
DOI: 10.24517/00050080
Source: https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=43738&item_no=1&attribute_id=26&file_no=1
https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=43738&item_no=1&attribute_id=26&file_no=1
Access Restrictions: 限定公開
Rights (production): CC BY-NC-ND
Related Material: https://kaken.nii.ac.jp/search/?qm=30377428
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500330/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-22500330/22500330seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA