肝炎ウイルス複製を支持する新規肝癌由来培養細胞の樹立

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Author: 本多, 政夫ほか

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Publication date: 2016-05-19

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Note (General)：: 金沢大学医薬保健研究域保健学系ゲノタイプ１ｂ型HCV 感染症例の肝細胞癌手術検体から癌幹細胞マーカーEpCAM陽性細胞分画を単離し、培養皿で継代が可能な細胞株（KH）を樹立した。KH細胞はJFH-1株やHJ3-5株のHCVの感染・複製を効率良く支持した。KH細胞はRIG-Iの変異を認めず、IFN誘導...

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https://kaken.nii.ac.jp/search/?qm=00272980

https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26670378/

https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26670378/26670378seika/

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Material Type: 文書・図像類
Title: 肝炎ウイルス複製を支持する新規肝癌由来培養細胞の樹立
Author/Editor: 本多, 政夫
Honda, Masao
Author Heading: 本多, 政夫
Honda, Masao
Publication Date: 2016-05-19
Publication Date (W3CDTF): 2016-05-19
Alternative Title: Establishment new HCC derived cell lines that support efficient hepatitis virus replication
Periodical title: 平成27(2015)年度科学研究費補助金挑戦的萌芽研究研究成果報告書 = 2015 Fiscal Year Final Research Report
No. or year of volume/issue: 2014-04-01 - 2016-03-31
Volume: 2014-04-01 - 2016-03-31
Pages: 6p.-
Text Language Code: jpn
Subject Heading: ウイルス培養細胞
幹細胞マーカー
自然免疫
癌
感染症
Target Audience: 一般
Note (General): 金沢大学医薬保健研究域保健学系
ゲノタイプ１ｂ型HCV 感染症例の肝細胞癌手術検体から癌幹細胞マーカーEpCAM陽性細胞分画を単離し、培養皿で継代が可能な細胞株（KH）を樹立した。KH細胞はJFH-1株やHJ3-5株のHCVの感染・複製を効率良く支持した。KH細胞はRIG-Iの変異を認めず、IFN誘導が保たれ、HCVの増殖は感染直後一端漸減し、再上昇を求めた。KH細胞はp53野生型であり、NOD-SCIDマウスに効率よく腫瘍を形成した。以上より、HCV感染・複製を支持する新規肝癌培養細胞株KH細胞を樹立した。KH細胞のゲノタイプ1bのHCV複製の確立には更なる検討が必要と考えられた。
We established a new cell line that is positive for cancer stem cell marker, EpCAM and supports efficient HCV replication and infection (JFH-1 and HJ3-5), from HCC tissue infected with genotype 1b HCV. KH cells have intact RIG-I sequence and produce IFN signaling, therefore, HCV replication once decline and increased again after infection. TP53 status of KH is wild and established tumor formation in NOD-SCID mice. Thus, we established a new HCC cell line that support efficient HCV replication and infection. Further studies should be needed to establish KH derived cells that support an efficient genotype 1b HCV replication.
研究課題/領域番号:26670378, 研究期間(年度):2014-04-01 - 2016-03-31
DOI: 10.24517/00050629
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=00272980
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26670378/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26670378/26670378seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA