宿主消化管内におけるボツリヌス毒素の動態解明

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Author: 油谷, 雅広ほか

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Publication date: 2017-06-01

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Note (General)：: 金沢大学医薬保健研究域医学系ボツリヌス菌が産生するボツリヌス神経毒素(BoNT)は、重篤な食中毒である食餌性ボツリヌス症を引き起こす。BoNTは無毒タンパク質(NAPs）と複合体を形成し、NAPの機能によって宿主腸管から効率よく吸収される。本複合体はpH 7.0以上の緩衝液中でBoNTとNAPsに解...

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Material Type: 文書・図像類
Title: 宿主消化管内におけるボツリヌス毒素の動態解明
Author/Editor: 油谷, 雅広
Yutani, Masahiro
Author Heading: 油谷, 雅広
Yutani, Masahiro
Publication Date: 2017-06-01
Publication Date (W3CDTF): 2017-06-01
Alternative Title: The study of molecular assembly of botulinum neurotoxin complex in host intestine
Periodical title: 平成28(2016)年度科学研究費補助金若手研究(B) 研究成果報告書 = 2016 Fiscal Year Final Research Report
No. or year of volume/issue: 2014-04-01 - 2017-03-31
Volume: 2014-04-01 - 2017-03-31
Pages: 6p.-
Text Language Code: jpn
Subject Heading: 細菌毒素
ボツリヌス症
食中毒
感染機構
Target Audience: 一般
Note (General): 金沢大学医薬保健研究域医学系
ボツリヌス菌が産生するボツリヌス神経毒素(BoNT)は、重篤な食中毒である食餌性ボツリヌス症を引き起こす。BoNTは無毒タンパク質(NAPs）と複合体を形成し、NAPの機能によって宿主腸管から効率よく吸収される。本複合体はpH 7.0以上の緩衝液中でBoNTとNAPsに解離することから、弱アルカリ性である宿主腸管でも解離すると考えられてきた。本研究では、宿主腸管に存在する宿主由来因子が、弱アルカリ性環境下で本複合体の解離を阻害することを明らかにした。このことは、宿主腸管内でも本毒素複合体が解離しないことを示唆しており、本研究によってボツリヌス食中毒の発症機構における重要な知見が得られた。
Clostridium botulinum neurotoxin (BoNT) exists as progenitor toxin complexes (PTCs) with nontoxic components such as hemagglutinin (HA) and non-toxic non-HA (NTNHA). In PTC molecules, BoNT associates with NTNHA noncovalently. NTNHA protects BoNT from digestion and HA facilitates BoNT absorption in host digestive tract.BoNT dissociates from NTNHA in weak alkaline buffer (pH > 7.0) in vitro, so that BoNT is assumed to be present as a released form in host intestine. Meanwhile, it is reported that the BoNT does not dissociate in rat intestinal fluid (pH 7.0) by sucrose gradient ultracentrifugation analysis. However, further studies were not performed.In this research, we found that the mouse intestinal fluid fraction, which contains heat-resistant small molecular weight compounds, inhibits the dissociation between BoNT and NAPs in alkaline conditions. This result suggests that BoNT does not dissociate from NAP in host intestinal tract.
研究課題/領域番号:26860287, 研究期間(年度):2014-04-01 - 2017-03-3
DOI: 10.24517/00050938
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=20648810
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26860287/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26860287/26860287seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA