胃癌組織分化に関わる遺伝子の網羅的探索

Material type: 文書・図像類

Author: 中村, 律子ほか

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Publication date: 2016-05-19

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Note (General)：: 金沢大学医薬保健研究域医学系単一胃癌内の分化度の異なる部位（管状腺癌および低分化腺癌）からRNAを抽出しマイクロアレイで解析した。発現に差がみられた遺伝子をqPCRで確認、マイクロアレイと発現傾向が一致した遺伝子を形態形成に関わる候補遺伝子と考えた。その中から、管状腺癌より低分化腺癌で発現が低下して...

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https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26860235/

https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26860235/26860235seika/

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Material Type: 文書・図像類
Title: 胃癌組織分化に関わる遺伝子の網羅的探索
Author/Editor: 中村, 律子
Nakamura, Ritsuko
Author Heading: 中村, 律子
Nakamura, Ritsuko
Publication Date: 2016-05-19
Publication Date (W3CDTF): 2016-05-19
Alternative Title: Comprehensive analysis of gene expression in different histological types of gastric cancer
Periodical title: 平成27(2015)年度科学研究費補助金若手研究(B) 研究成果報告書 = 2015 Fiscal Year Final Research Report
No. or year of volume/issue: 2014-04-01 - 2016-03-31
Volume: 2014-04-01 - 2016-03-31
Pages: 4p.-
Text Language Code: jpn
Subject Heading: 胃癌
組織型
Target Audience: 一般
Note (General): 金沢大学医薬保健研究域医学系
単一胃癌内の分化度の異なる部位（管状腺癌および低分化腺癌）からRNAを抽出しマイクロアレイで解析した。発現に差がみられた遺伝子をqPCRで確認、マイクロアレイと発現傾向が一致した遺伝子を形態形成に関わる候補遺伝子と考えた。その中から、管状腺癌より低分化腺癌で発現が低下していたHRASLS2 (HRAS like suppressor 2)に着目し、MKN45での安定発現株を作成したところ細胞接着・増殖形態に変化を及ぼした。
Gastric cancer has been classified according to histological types such as tubular adenocarcinoma and poorly differentiated adenocarcinoma. Two or three types are often presented in one tumor. The mechanisms to form the different histological types are not clear yet. To elucidate which molecules are related to morphological formation in gastric cancer, we performed microarray analysis of RNA from a formalin-fixed paraffin-embedded gastric cancer specimen which has both tubular adenocarcinoma and poorly differentiated adenocarcinoma. Several genes which showed different expression level between tubular adenocarcinoma and poorly differentiated adenocarcinoma by microarray were selected and confirmed the RNA expression level using qPCR. We focused on HRASLS2 (HRAS like suppressor 2) and created stable cell line expressing HRASLS2. Compare to the control, HRASLS2 expressing cell line showed different cell adhesion and cell growth pattern.
研究課題/領域番号:26860235, 研究期間(年度):2014-04-01 - 2016-03-31
DOI: 10.24517/00050998
Source: https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=44657&item_no=1&attribute_id=26&file_no=1
https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=44657&item_no=1&attribute_id=26&file_no=1
Access Restrictions: 限定公開
Rights (production): CC BY-NC-ND
Related Material: https://kaken.nii.ac.jp/search/?qm=20632657
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26860235/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26860235/26860235seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA