線虫をモデルとした光刺激と寿命・老化制御の分子基盤の解明

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Author: 原田, 真市ほか

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Publication date: 2017-06-08

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Note (General)：: 金沢大学医薬保健研究域医学系LED光源の中でも、特に青色LEDに着目し線虫の寿命に与える影響について検討した。青色LEDにより線虫の寿命は有意に短くなった。さらに青色LED曝露による線虫の活性酸素種を測定したところ僅かではあるが酵素活性の上昇が認められた。遺伝子発現解析からは、触感覚神経で発現するm...

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Material Type: 文書・図像類
Title: 線虫をモデルとした光刺激と寿命・老化制御の分子基盤の解明
Author/Editor: 原田, 真市
Harada, Shinichi
Author Heading: 原田, 真市
Harada, Shinichi
Publication Date: 2017-06-08
Publication Date (W3CDTF): 2017-06-08
Alternative Title: Molecular basis of lifespan and aging by exposing LED light in C. elegans
Periodical title: 平成28(2016)年度科学研究費補助金基盤研究(C) 研究成果報告書 = 2016 Fiscal Year Final Research Report
No. or year of volume/issue: 2014-04-01 - 2017-03-31
Volume: 2014-04-01 - 2017-03-31
Pages: 5p.-
Text Language Code: jpn
Subject Heading: C. elegans
LED light
Target Audience: 一般
Note (General): 金沢大学医薬保健研究域医学系
LED光源の中でも、特に青色LEDに着目し線虫の寿命に与える影響について検討した。青色LEDにより線虫の寿命は有意に短くなった。さらに青色LED曝露による線虫の活性酸素種を測定したところ僅かではあるが酵素活性の上昇が認められた。遺伝子発現解析からは、触感覚神経で発現するmec-5遺伝子や神経系カルシウムセンサーとして機能すると考えられるncs-1遺伝子の発現が有意に増減していた。また線虫の行動解析からはコントロールに比べ青色LED照射により行動距離が短くなった。以上のことから線虫では青色LED曝露により酸化ストレスが上昇し、行動が抑制されることが明らかとなった。
To invesigate the biological disadvantage of blue LED light, we used the free-living nematode Caenorhabditis elegans, as a model. By exposing blue LED light at 465nm, the lifespan of C. elegans was decreased, and these reactive oxygen species (ROS) activity as a marker of stress response were slightly increased. Using Real-Time RT-PCR analysis to measure gene expression levels by exposure of LED light, we found that mec-5 gene which express in sensory neuron was highly expressed and neural calcium sensor (ncs-1) gene was significantly decreased in worms with blue LED light as compared to dark exposure as a control. Furthermore, we found to cause a marked deterioration in the worm's behavior with blue LED light. It is concluded that exposure of blue LED light may cause oxidative stress, and result in abnormal locomotion and survival in the nematode C. elegans. However, further investigation is required to clarify the toxicological effect of the LEDs light.
研究課題/領域番号:26430068, 研究期間(年度):2014-04-01 - 2017-03-31
DOI: 10.24517/00051593
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=90272955
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26430068/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26430068/26430068seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA