植物の抵抗性系統のスタッキングによるカビ毒産生菌に対する防除戦略の確立

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Author: 西内, 巧ほか

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Publication date: 2017-06-12

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Note (General)：: 金沢大学学際科学実験センター遺伝子研究施設植物病原糸状菌であるムギ類赤かび病菌は、作物に減収だけでなくかび毒汚染による質的な被害をもたらす。本研究では、本菌に罹病性のシロイヌナズナを用いて、植物の抵抗性やかび毒解毒に関わる候補遺伝子の発現を改変させた形質転換体を解析し、抵抗性を顕著に向上させる2遺伝...

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https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26450053/26450053seika/

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Material Type: 文書・図像類
Title: 植物の抵抗性系統のスタッキングによるカビ毒産生菌に対する防除戦略の確立
Author/Editor: 西内, 巧
Nishiuchi, Takumi
Author Heading: 西内, 巧
Nishiuchi, Takumi
Publication Date: 2017-06-12
Publication Date (W3CDTF): 2017-06-12
Alternative Title: Transgenic stacking to improve plant disease resistance against mycotoxin-producing fungi
Periodical title: 平成28(2016)年度科学研究費補助金基盤研究(C) 研究成果報告書 = 2016 Fiscal Year Final Research Report
No. or year of volume/issue: 2014-04-01 - 2017-03-31
Volume: 2014-04-01 - 2017-03-31
Pages: 6p.-
Text Language Code: jpn
Subject Heading: 病害抵抗性
トリコテセン系かび毒
マイコトキシン
赤かび病
スタッキング
解毒
Target Audience: 一般
Note (General): 金沢大学学際科学実験センター遺伝子研究施設
植物病原糸状菌であるムギ類赤かび病菌は、作物に減収だけでなくかび毒汚染による質的な被害をもたらす。本研究では、本菌に罹病性のシロイヌナズナを用いて、植物の抵抗性やかび毒解毒に関わる候補遺伝子の発現を改変させた形質転換体を解析し、抵抗性を顕著に向上させる2遺伝子、かび毒を低減化させる2遺伝子を同定した。これらの遺伝子を効果的に組合せることで、赤かび病による病徴とかび毒蓄積を共に低減化し得る植物の作出が期待された。
Fusarium head blight (FHB) is serious disease leading to significant yield loss and accumulation of trichothecene mycotoxins. To improve disease resistance and mycotoxin detoxification in plants against F. graminearum, we generated transgenic stacking in Arabidopsis plants. We had identified some defense-related genes that are involved in the disease resistance against F. graminearum. Among these genes, we revealed that the ubq6/6* double mutant strong disease resistance. The barley HvGST13 and HvGR2 genes were specifically upregulated in the barley breeding lines showing lower mycotoxin (DON) accumulation. The HvGST13ox and HvGR2ox Arabidopsis plants were DON-resistant phenotypes compared to wild type. In addition, HvGST13ox Arabidopsis plants showed weak disease symptoms and low DON accumulation after inoculation of F. graminearum conidia. In the future, crossing the ubq6/6* mutant with HvGST13ox and HvGR2ox likely shows low disease symptoms and DON accumulation by F. graminearum.
研究課題/領域番号:26450053, 研究期間(年度):2014-04-01 - 2017-03-31
DOI: 10.24517/00052415
Source: https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=46081&item_no=1&attribute_id=26&file_no=1
https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=46081&item_no=1&attribute_id=26&file_no=1
Access Restrictions: 限定公開
Rights (production): CC BY-NC-ND
Related Material: https://kaken.nii.ac.jp/search/?qm=20334790
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26450053/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26450053/26450053seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA