新規病原ウイルス単離の試み

Material type: 文書・図像類

Author: 村松, 正道ほか

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Publication date: 2016-05-31

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Note (General)：: 金沢大学医薬保健研究域医学系日和見感染時に病原性を示すウイルスのうち環状DNAをゲノムにもつものが特にポリオーマウイルスで報告されている。そこでDNA抽出産物から環状DNAを強力に増幅する手法で臨床検体中に新規病原体DNAが含まれるかを検討した。既存のパピローマウイルスは期待通り増幅されたが、新種の...

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Material Type: 文書・図像類
Title: 新規病原ウイルス単離の試み
Author/Editor: 村松, 正道
Muramatsu, Masamichi
Author Heading: 村松, 正道
Muramatsu, Masamichi
Publication Date: 2016-05-31
Publication Date (W3CDTF): 2016-05-31
Alternative Title: A trial to isolate novel pathogenic viruses
Periodical title: 平成27(2015)年度科学研究費補助金挑戦的萌芽研究研究成果報告書 = 2015 Fiscal Year Final Research Report
No. or year of volume/issue: 2014-04-01 - 2016-03-31
Volume: 2014-04-01 - 2016-03-31
Pages: 4p.-
Text Language Code: jpn
Subject Heading: Circular DNA
virus
Polyomavirus
Target Audience: 一般
Note (General): 金沢大学医薬保健研究域医学系
日和見感染時に病原性を示すウイルスのうち環状DNAをゲノムにもつものが特にポリオーマウイルスで報告されている。そこでDNA抽出産物から環状DNAを強力に増幅する手法で臨床検体中に新規病原体DNAが含まれるかを検討した。既存のパピローマウイルスは期待通り増幅されたが、新種のウイルスはみつからなかった。しかし由来不明の環状DNAを見つけた。由来不明DNA同定は病原性は不明だが一定の成果と言える。
Recently, many novel polyomaviruses have been identified as a candidate pathogenic virus that is responsible for particular diseases in immune compromised patients. This study has tried to isolate novel viruses responsible for a disease by rolling cycle amplification which can strongly amplify viral circular genomic DNA. Using test samples, we set up a condition where we can amplify a very small amount of circular DNA from total DNA sample. Then, we tested series of healthy- and disease- derived samples to amplify circular DNA. Most of amplified DNA are either artifacts or mitochondrial DNA. Skin samples provide amplification of MCV polyomavirus, that is consistent with previous published reports. However, we do not find a novel polyomavirus. Origin unknown circular DNA are also found, which is a clear outcome of this study, in spit of few information of pathogenicity.
研究課題/領域番号:26670223, 研究期間(年度):2014-04-01 - 2016-03-31
DOI: 10.24517/00054886
Source: https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=48564&item_no=1&attribute_id=26&file_no=1
https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=48564&item_no=1&attribute_id=26&file_no=1
Access Restrictions: 限定公開
Rights (production): CC BY-NC-ND
Related Material: https://kaken.nii.ac.jp/search/?qm=20359813
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26670223/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26670223/26670223seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA