婦人科癌におけるテロメラーゼ活性化機転に基づく末梢血腫瘍細胞の検出・解析法の確立

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Author: 高倉, 正博ほか

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Publication date: 2017-06-08

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Note (General)：: 金沢大学附属病院テロメラーゼ活性を指標として末梢血中の癌細胞を検出・解析するためにhTERTプロモーター活性化条件においてのみ増殖しGFPを発現する制限増殖型アデノウイルスベクターを用いた。子宮頸癌患者より検出したCTCに対しサイトケラチンの発現解析後、顕微鏡下に回収、CTCから原発巣と同じHPV ...

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https://kaken.nii.ac.jp/search/?qm=20313661

https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26462516/

https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26462516/26462516seika/

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Material Type: 文書・図像類
Title: 婦人科癌におけるテロメラーゼ活性化機転に基づく末梢血腫瘍細胞の検出・解析法の確立
Author/Editor: 高倉, 正博
Takakura, Masahiro
Author Heading: 高倉, 正博
Takakura, Masahiro
Publication Date: 2017-06-08
Publication Date (W3CDTF): 2017-06-08
Alternative Title: Application of telomerase activation mechanism to detect and isolate circulating tumor cells in patients with gynecologic cancers
Periodical title: 平成28(2016)年度科学研究費補助金基盤研究(C) 研究成果報告書 = 2016 Fiscal Year Final Research Report
No. or year of volume/issue: 2014-04-01 - 2017-03-31
Volume: 2014-04-01 - 2017-03-31
Pages: 4p.-
Text Language Code: jpn
Subject Heading: 末梢血中腫瘍細胞
テロメラーゼ
子宮頸癌
HPV
Target Audience: 一般
Note (General): 金沢大学附属病院
テロメラーゼ活性を指標として末梢血中の癌細胞を検出・解析するためにhTERTプロモーター活性化条件においてのみ増殖しGFPを発現する制限増殖型アデノウイルスベクターを用いた。子宮頸癌患者より検出したCTCに対しサイトケラチンの発現解析後、顕微鏡下に回収、CTCから原発巣と同じHPV DNAを検出し癌細胞由来であることを確認した。子宮頸癌でのCTC検出率は約30%であり、臨床進行期などとの相関は認めなかった。回収されたCTCはすべてサイトケラチン陰性であった。このことは本方法で検出したCTCはCellSearch等の上皮マーカー依存的な検出法では見逃されていたものであることを示している。
We applied conditional replication-selective adenovirus vector (modified TRAD) which replicate and express GFP only in cells which hTERT (human telomerase reverse transcriptase) promoter is activated to detect circulating tumor cells (CTC). The blood from cervical cancer patients was infected with modified TRAD. GFP expressing cells were performed immunocytological analysis for cytokeratins and isolated under microscopic observation. The isolated cells were subjected to whole genome amplification followed by a PCR analysis of HPV DNA identical with their primary lesion proving they were originated from cancer.CTC were detected in about 30% of cervical cancer patients. There was no correlation with tumor stage or other clinicopathological factors. All CTCs we detected were cytokeratin negative, suggesting CTC detecting method depending epithelial marker such as CellSearch may overlook them. It might be important to analyze these cells to reveal the mechanisms of cancer metastasis.
研究課題/領域番号:26462516, 研究期間(年度):2014-04-01 - 2017-03-31
DOI: 10.24517/00056930
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=20313661
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26462516/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26462516/26462516seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA