胃癌におけるCCND1, CCNE1及びCDK6 遺伝子増幅の検索

Material type: 文書・図像類

Author: 大井, 章史ほか

Publisher: 金沢大学医薬保健研究域医学系

Publication date: 2019-05-21

Material Format: Digital

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Note (General)：: 増幅遺伝子はしばしば分子標的の対象となる。本研究では、胃癌及び乳癌において、遺伝子増幅の報告のあるそれぞれ16遺伝子、22遺伝子についてmuliple ligation-dependent probe amplification とfluorescence in situ hybridization...

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https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K08686/

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Material Type: 文書・図像類
Title: 胃癌におけるCCND1, CCNE1及びCDK6 遺伝子増幅の検索
Author/Editor: 大井, 章史
Ooi, Akishi
Author Heading: 大井, 章史
Ooi, Akishi
Publication, Distribution, etc.: 金沢大学医薬保健研究域医学系
Publication Date: 2019-05-21
Publication Date (W3CDTF): 2019-05-21
Alternative Title: Analysis of gene amplifications of CCND1, CCNE1, and CDK6 in gastric cancers
Periodical title: 平成30(2018)年度科学研究費補助金基盤研究(C) 研究成果報告書 = 2018 Fiscal Year Final Research Report
No. or year of volume/issue: 2016-04-01 - 2019-03-31
Volume: 2016-04-01 - 2019-03-31
Pages: 4p.-
Text Language Code: jpn
Subject Heading: 胃癌
乳癌
遺伝子増幅
MLPA
FISH
Target Audience: 一般
Note (General): 増幅遺伝子はしばしば分子標的の対象となる。本研究では、胃癌及び乳癌において、遺伝子増幅の報告のあるそれぞれ16遺伝子、22遺伝子についてmuliple ligation-dependent probe amplification とfluorescence in situ hybridizationを用いて増幅を検索した。胃癌ではCCNE１, CCND１、CDK４遺伝子とERBB2, EGFR, KRAS遺伝子との同時増幅が高頻度にみられた。また乳癌では8p11-12、11q11.3 と17q12-21上で複数の遺伝子増幅がおこっており、8p11 と 11q13上の遺伝子の同時増幅がみられた。
In solid cancers amplified genes are potential targets of molecular therapies. In this study the 16 genes and 22 genes reportedly amplified in gastric and mammary cancers were examined by multiplex ligation-dependent probe amplification and fluorescence in situ hybridization.　In gastric cancers amplification of G1-S regulatory genes (CCNE1, CCND1and CDK6) were co-amplified with ERBB2, EGFR and/or KRAS in single cancer nuclei. In breast cancers The frequencies of the amplification of four regions containing known driver oncogenes were as follows: 8p11 (ZNF703, FGFR1, ADAM9 and IKBKB), 8q24 (MYC), 11q13 (CCND1, C11ORF30), and 17q11-21 (CPD, MED1, ERBB2, CDC6, TOP2A, MAPT) exhibited amplification in 9.6%, 9.6%, 12.4%, and 12.1% of the tumors, respectively. Co-localization of the amplicon on 8p11 and the amplicon on 11q13 in single cells was frequently observed. Precise and feasible analysis of gene amplification status can be obtained using a combination of MLPA and FISH.
研究課題/領域番号:16K08686, 研究期間(年度):2016-04-01 - 2019-03-31
出典：「胃癌におけるCCND1, CCNE1及びCDK6 遺伝子増幅の検索」研究成果報告書　課題番号16K08686（KAKEN：科学研究費助成事業データベース（国立情報学研究所））（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K08686/16K08686seika/）を加工して作成
DOI: 10.24517/00052868
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=50160411
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K08686/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K08686/16K08686seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA