精細管の三次元構造と精子形成能の空間的「偏り」の解明

Material type: 文書・図像類

Author: 仲田, 浩規ほか

Publisher: 金沢大学医薬保健研究域医学系

Publication date: 2019-05-13

Material Format: Digital

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Note (General)：: 蛍光染色またはPAS染色を用いた半自動の三次元再構築方法を考案し、0日齢、21日齢、70日齢の精細管の三次元再構築を各3例行い、生後発達におけるマウス精細管の本数・分岐・長さ・走行・相互関係を明らかにした。また、精子形成が開始した場所と精子形成の波を三次元で明らかにし、精子形成に関わる空間的な偏りを...

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https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K18976/

https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K18976/16K18976seika/

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Material Type: 文書・図像類
Title: 精細管の三次元構造と精子形成能の空間的「偏り」の解明
Author/Editor: 仲田, 浩規
Nakata, Hiroki
Author Heading: 仲田, 浩規
Nakata, Hiroki
Publication, Distribution, etc.: 金沢大学医薬保健研究域医学系
Publication Date: 2019-05-13
Publication Date (W3CDTF): 2019-05-13
Alternative Title: Three-dimensional structure and analysis of seminiferous tubules
Periodical title: 平成30(2018)年度科学研究費補助金若手研究(B) 研究成果報告書 = 2018 Fiscal Year Final Research Report
No. or year of volume/issue: 2016-04-01 - 2019-03-31
Volume: 2016-04-01 - 2019-03-31
Pages: 4p.-
Text Language Code: jpn
Subject Heading: 精巣
精細管
三次元
三次元再構築
マウス
3D
Target Audience: 一般
Note (General): 蛍光染色またはPAS染色を用いた半自動の三次元再構築方法を考案し、0日齢、21日齢、70日齢の精細管の三次元再構築を各3例行い、生後発達におけるマウス精細管の本数・分岐・長さ・走行・相互関係を明らかにした。また、精子形成が開始した場所と精子形成の波を三次元で明らかにし、精子形成に関わる空間的な偏りを明らかにした。
We developed a technique to analyze the high-resolution three-dimensional (3D) structure of seminiferous tubules. It consists of the segmentation of tubules in serial paraffin sections of the testis by marking the basement membrane with periodic acid-Schiff or a fluorescent anti-laminin antibody followed by the 3D reconstruction of tubules with high-performance software. Using this method, we analyzed testes from mice at different ages and accurately elucidated the 3D structure of seminiferous tubules, including the number and length of tubules as well as the numbers of connections with the rete testis, branching points, and blind ends. We also analyzed the distribution and direction of spermatogenic waves along the length of adult seminiferous tubules as well as the site of the first onset of spermatogenesis in postnatal seminiferous tubules.
研究課題/領域番号:16K18976, 研究期間(年度):2016-04-01 - 2019-03-31
出典：研究課題「精細管の三次元構造と精子形成能の空間的「偏り」の解明」課題番号16K18976（KAKEN：科学研究費助成事業データベース（国立情報学研究所））（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K18976/16K18976seika/）を加工して作成
DOI: 10.24517/00055122
Source: https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=48802&item_no=1&attribute_id=26&file_no=1
https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=48802&item_no=1&attribute_id=26&file_no=1
Access Restrictions: 限定公開
Rights (production): CC BY-NC-ND
Related Material: https://kaken.nii.ac.jp/search/?qm=80638304
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K18976/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K18976/16K18976seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA