糖尿病における心・腎線維化機序に関わる新規骨髄由来細胞の同定と解析

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Author: 相良, 明宏ほか

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Publication date: 2017-05-23

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Note (General)：: 金沢大学附属病院全身GFP発現マウスの骨髄を野生型B6マウスに移植し，一側尿管結紮（UUO）を行うことで腎線維化モデルを作成した．心，腎に浸潤したGFP+CD45+Sca1+細胞に着目し，Fibroblastsとの共培養やCOL-GFPマウスを用いた骨髄移植による検討にて，CD45+Sca1+細胞の...

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Material Type: 文書・図像類
Title: 糖尿病における心・腎線維化機序に関わる新規骨髄由来細胞の同定と解析
Author/Editor: 相良, 明宏
Sagara, Akihiro
Author Heading: 相良, 明宏
Sagara, Akihiro
Publication Date: 2017-05-23
Publication Date (W3CDTF): 2017-05-23
Alternative Title: Identification of new myeloid-derived fibrosis-inducing cells accounting for cardiorenal connection in diabetic nephropathy
Periodical title: 平成28(2016)年度科学研究費補助金若手研究(B) 研究成果報告書 = 2016 Fiscal Year Final Research Report
No. or year of volume/issue: 2015-04-01 – 2017-03-31
Volume: 2015-04-01 – 2017-03-31
Pages: 4p.-
Text Language Code: jpn
Subject Heading: 骨髄由来細胞
繊維化
心腎連関
Target Audience: 一般
Note (General): 金沢大学附属病院
全身GFP発現マウスの骨髄を野生型B6マウスに移植し，一側尿管結紮（UUO）を行うことで腎線維化モデルを作成した．心，腎に浸潤したGFP+CD45+Sca1+細胞に着目し，Fibroblastsとの共培養やCOL-GFPマウスを用いた骨髄移植による検討にて，CD45+Sca1+細胞の線維化誘導能とコラーゲン産生能を確認した．また CD45+Sca1+細胞よりRNAを抽出し，Genechipによる遺伝子発現解析を行った．共培養とフローサイトメトリーによる検証を経て，CD45+Sca1+細胞の中に更なる亜集団が存在することを示した．この細胞群はヒト末梢血にも存在し，腎機能の増悪に伴って増加した．
To chase and identify fibrosis-related cell types, we used unilateral ureteral obstruction model mice, which showed renal and heart fibrosis, in combination with GFP-based tracing systems such as bone-marrow transplantation (BMT). We found BM-derived mononuclear cell cluster of CD45+Sca1+ cells mobilized and accumulated in the fibrotic lesions of kidney and heart using flow cytometry (FCM). The CD45+Sca1+ cells had an activating potential for collagen production of cultured fibroblasts and also produced type 1 collagen by themselves. Genechip analyses and FCM revealed the subpopulation of CD45+Sca1+ cells, which were related to chemotaxis and innate immunity. These cells were also detected in human peripheral blood and increased in proportion to kidney dysfunction. In this study, we succeeded to identify a new myeloid-derived fibrosis-inducing cells, which could provide a new avenue in fibrosis.
研究課題/領域番号:15K19449, 研究期間(年度):2015-04-01 – 2017-03-31
DOI: 10.24517/00058820
Source: https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=52526&item_no=1&attribute_id=26&file_no=1
https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=52526&item_no=1&attribute_id=26&file_no=1
Access Restrictions: 限定公開
Rights (production): CC BY-NC-ND
Related Material: https://kaken.nii.ac.jp/ja/search/?kw=00707060
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-15K19449/
https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-15K19449/15K19449seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA