DNAメチル化異常による精子形成障害と不妊治療に及ぼす影響

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Author: 杉本, 和宏ほか

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Publication date: 2014-05-22

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Note (General)：: 金沢大学医薬保健研究域医学系エピジェネティック制御と男性不妊症との関係に関するエビデンスは増えている。今回我々は、プロモーター領域にTDMR（組織特異的メチル化可変領域）をもつGTF2A1L遺伝子に注目して研究を行った。86症例の非閉塞性無精子症の中で17症例がhypospermatogenesis...

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Material Type: 文書・図像類
Title: DNAメチル化異常による精子形成障害と不妊治療に及ぼす影響
Author/Editor: 杉本, 和宏
Sugimoto, Kazuhiro
Author Heading: 杉本, 和宏
Sugimoto, Kazuhiro
Publication Date: 2014-05-22
Publication Date (W3CDTF): 2014-05-22
Alternative Title: Spermatogenesis disturbance due to aberrant DNA methylation and its impact on fertility treatment
Periodical title: 平成25(2013)年度科学研究費補助金若手研究(B) 研究成果報告書 = 2013 Fiscal Year Final Research Report
No. or year of volume/issue: 2012-04-01 – 2014-03-31
Volume: 2012-04-01 – 2014-03-31
Pages: 4p.-
Text Language Code: jpn
Subject Heading: エピジェネティクス
メチル化
不妊症
精子
Target Audience: 一般
Note (General): 金沢大学医薬保健研究域医学系
エピジェネティック制御と男性不妊症との関係に関するエビデンスは増えている。今回我々は、プロモーター領域にTDMR（組織特異的メチル化可変領域）をもつGTF2A1L遺伝子に注目して研究を行った。86症例の非閉塞性無精子症の中で17症例がhypospermatogenesisの組織型であった。この中で、5例がTDMRの高メチル化群、12例が低メチル化群であった。TDMRの高メチル化は、GTF2A1L遺伝子発現の低下と関連していた。しかし、両群とも精子回収率、受精率、妊娠率、出生率に関して比較的好成績であり、GTF2A1L遺伝子発現の異常は妊娠率へは影響を与えていなかった。
The GTF2A1L gene promoter contains the DNA methylation site of a tissue-specific differentially methylated region (TDMR). Eighty-six patients with non-obstructive azoospermia were assessedfor the DNA methylation state of CpG islands in the GTF2A1L promoter using testicular genomic DNA. Based on histological criteria, 17 of the 86 patients had hypospermatogenesis. Patients with hypospermatogenesis were divided into two subgroups: high DNA methylation(HM, n=5) and low DNA methylation (LM, n=12). The GTF2A1L TDMR methylation rate differed significantly between the HM and LM groups (P=0.0019), and GTF2A1L expression was significantly higher among the LM than in the HM patients (P=0.023). High TDMR methylation was correlated with low GTF2A1L gene expression levels. Both groups demonstrated relatively good outcomes with respect to sperm retrieval, fertilisation, pregnancy and childbirth rates. We observed that aberrant GTF2A1L gene expression was not correlated with fertilisation rates.
研究課題/領域番号:24791638, 研究期間(年度):2012-04-01 – 2014-03-31
DOI: 10.24517/00059694
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/ja/search/?kw=70621837
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-24791638
https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-24791638/24791638seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA