骨肉腫の肺転移形成と血液凝固・線溶因子との関連

Material type: 文書・図像類

Author: 木村, 浩明ほか

Publisher: -

Publication date: 2013-05-20

Material Format: Digital

Capacity, size, etc.: -

NDC: -

View All

Notes on use

Note (General)：: 金沢大学医薬保健研究域医学系医学系ヒト骨肉腫cell line "143B" の核にGFP、細胞質にRFPが発現した2色の蛍光を有する143B-dualを使用し、転移能の異なる複数のcell lineを最初に樹立した。それらの細胞におけるuPA、PAI-1、TFの発現量をElizaとPCRで確認し、...

Related materials as well as pre- and post-revision versions

https://kaken.nii.ac.jp/search/?qm=50608693

https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23890070/

https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23890070/23890070seika/

Search by Bookstore

Holdings of Libraries in Japan

This page shows libraries in Japan other than the National Diet Library that hold the material.

List of Cooperating Institutions and Databases

Please contact your local library for information on how to use materials or whether it is possible to request materials from the holding libraries.

other

Kanazawa University Repository for Academic Resources
Digital
You can check the holdings of institutions and databases with which Institutional Repositories DataBase(IRDB)(Institutional Repository) is linked at the site of Institutional Repositories DataBase(IRDB)(Institutional Repository).
Kanazawa University Repository for Academic Resources

Search by Bookstore

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 文書・図像類
Title: 骨肉腫の肺転移形成と血液凝固・線溶因子との関連
Author/Editor: 木村, 浩明
Kimura , Hiroaki
Author Heading: 木村, 浩明
Kimura , Hiroaki
Publication Date: 2013-05-20
Publication Date (W3CDTF): 2013-05-20
Alternative Title: Relationship between lung metastases of osteosarcoma and coagulation-fibrinolytic factors
Periodical title: 平成24(2012)年度科学研究費補助金研究活動スタート支援研究成果報告書 = 2012 Fiscal Year Final Research Report
No. or year of volume/issue: 2011 – 2012
Volume: 2011 – 2012
Pages: 5p.-
Text Language Code: jpn
Subject Heading: 骨肉腫
肺転移
プラスミノーゲン活性化因子インヒビタ-1 (PAI-1)
ウロキナーゼ型プラスミノ-ゲン活性化因子
蛍光蛋白
Target Audience: 一般
Note (General): 金沢大学医薬保健研究域医学系医学系
ヒト骨肉腫cell line "143B" の核にGFP、細胞質にRFPが発現した2色の蛍光を有する143B-dualを使用し、転移能の異なる複数のcell lineを最初に樹立した。それらの細胞におけるuPA、PAI-1、TFの発現量をElizaとPCRで確認し、uPAとPAI-1が転移能と強く関連している事が証明された。現在は143BにおけるuPAとPAI-1のshRNAを行い、in vivoにおいて転移能がどのように変化するかを観察する予定である。
With use of human osteosarcoma cell line “143B dual color”, in which GFP is expressing in nucleus and RFP is expressing in cytoplasm, we first developed multiple cell lines that have multiple metastatic potential. In each cell line, we evaluated expression level of uPA, PAI-1, and TF by ELIZA and PCR. The result showed strong relationship between metastatic potential and expression of uPA & PAI-1. We have now performed shRNA of uAP & PAI-1 for 143B cell line. Then we will evaluate how metastatic potential change after suppression of uPA and PAI-1 in osteosarcoma.
研究課題/領域番号:23890070, 研究期間(年度):2011-2012
DOI: 10.24517/00059879
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=50608693
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23890070/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23890070/23890070seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA