骨軟部腫瘍切除後欠損に対する脂肪由来幹細胞再生医療の新たなアプローチ

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Author: 林, 克洋ほか

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Publication date: 2020-05-18

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Note (General)：: 金沢大学医薬保健学総合研究科脂肪由来幹細胞（ADSC）の骨腫瘍切除後の欠損に対する骨再生効果を確認した。in vitroにおいて、ADSCによる凍結自家処理骨再生能を検討し骨芽細胞のSmadシグナルやJNKシグナルが有意に活性化、骨形成を促す液性因子を産生している可能性が確認され、in vivo実験...

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Material Type: 文書・図像類
Title: 骨軟部腫瘍切除後欠損に対する脂肪由来幹細胞再生医療の新たなアプローチ
Author/Editor: 林, 克洋
Hayashi, Katsuhiro
Author Heading: 林, 克洋
Hayashi, Katsuhiro
Publication Date: 2020-05-18
Publication Date (W3CDTF): 2020-05-18
Alternative Title: A new approach of adipose-derived stem cell regenerative medicine for postoperative defects after bone and soft tissue tumor surgery
Periodical title: 令和1(2019)年度科学研究費補助金基盤研究(C) 研究成果報告書 = 2019 Fiscal Year Final Research Report
No. or year of volume/issue: 2017-04-01 - 2020-03-31
Volume: 2017-04-01 - 2020-03-31
Pages: 6p.-
Text Language Code: jpn
Subject Heading: 脂肪由来幹細胞
骨軟部腫瘍
再生医療
Orthopaedic surgery
Target Audience: 一般
Note (General): 金沢大学医薬保健学総合研究科
脂肪由来幹細胞（ADSC）の骨腫瘍切除後の欠損に対する骨再生効果を確認した。in vitroにおいて、ADSCによる凍結自家処理骨再生能を検討し骨芽細胞のSmadシグナルやJNKシグナルが有意に活性化、骨形成を促す液性因子を産生している可能性が確認され、in vivo実験モデルで、凍結自家処理骨にADSCを添加することで、骨再生の亢進が確認された。In vitroでのBMPの発現効果やDiIの分布などとあわせ、ADSCは骨に直接分化したより、周囲に存在して骨形成サイトカインを分泌し骨形成を促進したと考えられた。今後ADSC添加による臨床応用が期待される。
Adipose-derived stem cells (ADSCs) have multipotentiality equivalent to bone marrow derived stem cells. We examined the ability of ADSCs to revitalized frozen bone toward clinical application of bone tumor surgery. In a conditioned culture method, proliferation and signaling pathways (Smad and JNK) of osteoblasts affected by ADSCs were examined using cell proliferation and Western blotting assays, and they were significantly enhanced. The methods confirmed osteoblast differentiation and osteogenesis-promoting action of ADSCs. Next, the femoral shafts of rats were excised, frozen in liquid nitrogen, and then reimplanted as an autologous bone transplant. Histopathologic examination was performed to assess its revitalization ability, and bone revitalization was observed significantly more with than without ADSCs in week 8. The osteogenesis-promoting action of ADSCs for frozen bone was suggested. Using ADSCs to promote revitalization of frozen bone is expected in the future.
研究課題/領域番号:17K10962, 研究期間(年度):2017-04-01 - 2020-03-31
出典：「骨軟部腫瘍切除後欠損に対する脂肪由来幹細胞再生医療の新たなアプローチ」研究成果報告書　課題番号17K10962（KAKEN：科学研究費助成事業データベース（国立情報学研究所））（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K10962/17K10962seika/)を加工して作成
DOI: 10.24517/00057935
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=80507054
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K10962/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K10962/17K10962seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA