PUVA処理した制御性樹状細胞の更なる免疫抑制増強の試み: 新たなGVHD治療戦略

Material type: 文書・図像類

Author: 前馬, 秀昭ほか

Publisher: -

Publication date: 2020-05-25

Material Format: Digital

Capacity, size, etc.: -

NDC: -

View All

Notes on use

Note (General)：: 金沢大学附属病院PUVA処理（Psoralen+UVA照射2J）した樹状細胞(PUVA-DC)がin vitroにおいて、制御性樹状細胞の性質を獲得したことを報告してきた．その細胞をマウスGVHDモデルにて検討を行ったが、GVHD抑制作用が得られなかったため、PUVA-DCの作製条件の検討を行った。...

Related materials as well as pre- and post-revision versions

https://kaken.nii.ac.jp/search/?qm=10419335

https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K10135/

https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K10135/17K10135seika/

Search by Bookstore

Holdings of Libraries in Japan

This page shows libraries in Japan other than the National Diet Library that hold the material.

List of Cooperating Institutions and Databases

Please contact your local library for information on how to use materials or whether it is possible to request materials from the holding libraries.

other

Kanazawa University Repository for Academic Resources
Digital
You can check the holdings of institutions and databases with which Institutional Repositories DataBase(IRDB)(Institutional Repository) is linked at the site of Institutional Repositories DataBase(IRDB)(Institutional Repository).
Kanazawa University Repository for Academic Resources

Search by Bookstore

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 文書・図像類
Title: PUVA処理した制御性樹状細胞の更なる免疫抑制増強の試み: 新たなGVHD治療戦略
Author/Editor: 前馬, 秀昭
Maeba, Hideaki
Author Heading: 前馬, 秀昭
Maeba, Hideaki
Publication Date: 2020-05-25
Publication Date (W3CDTF): 2020-05-25
Alternative Title: Improvement in making methods of PUVA-treated dendritic cells
Periodical title: 令和1(2019)年度科学研究費補助金基盤研究(C) 研究成果報告書 = 2019 Fiscal Year Final Research Report
No. or year of volume/issue: 2017-04-01 – 2020-03-31
Volume: 2017-04-01 – 2020-03-31
Pages: 8p.-
Text Language Code: jpn
Subject Heading: GVHD
psoralen
UVA
制御性樹状細胞
Pediatrics
Target Audience: 一般
Note (General): 金沢大学附属病院
PUVA処理（Psoralen+UVA照射2J）した樹状細胞(PUVA-DC)がin vitroにおいて、制御性樹状細胞の性質を獲得したことを報告してきた．その細胞をマウスGVHDモデルにて検討を行ったが、GVHD抑制作用が得られなかったため、PUVA-DCの作製条件の検討を行った。UVA照射の設定（0-2 J）を探索したが、0.2-1Jでは免疫寛容を有していなかった。次にカスパーゼ阻害剤による処理を追加したが、PUVA-DCの24時間後の生存率は改善せず免疫寛容誘導能も有していなかった．PUVA-DCを、細胞治療に用いるためには、さらなる培養条件の検討が必要である．
We have reported that bone marrow-derived dendritic cells (BM-DCs), which are generated from bone marrow cells plus GM-CSF, acquired tolerogenicity by PUVA-treatment(psoralen+UVA 2J/cm2) in mice.The PUVA-treated DCs have tolerogenic function in a MHC-independent manner. But these cells did not have the inhibitory effects in the mouse GVHD model. We tried to improve the manufacture condition of PUVA-DC, to make these cells engraft effectively in vivo. At first, we researched the setting (0-2 J /cm2) of the UVA radiation, but did not have acquired tolerance in the UVA 0.2-1J/cm2 setting. Next, we added caspase inhibitor to PUVA treatment for apoptotic suppression by the UVA radiation. The survival rate 24 hours after PUVA-treatment were not improved, and these cells did not have the acquired more tolerance than conventional PUVA-DC. The examination of the further culture condition is necessary to use PUVA-DC for cell therapy.
研究課題/領域番号:17K10135, 研究期間(年度):2017-04-01 – 2020-03-31
出典：「PUVA処理した制御性樹状細胞の更なる免疫抑制増強の試み: 新たなGVHD治療戦略」研究成果報告書　課題番号17K10135（KAKEN：科学研究費助成事業データベース（国立情報学研究所））（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K10135/17K10135seika/)を加工して作成
DOI: 10.24517/00058422
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=10419335
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17K10135/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K10135/17K10135seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA