微弱電磁場と不凍糖タンパクを用いた低温心保存法

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Author: 鷹合, 真太郎ほか

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Publication date: 2021-04-28

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Note (General)：: 金沢大学附属病院心臓血管外科ラット心を低温単純浸漬法にて保存実験した．control群（n=6）として保存液のみ，AFGP添加群（n=6）として保存液にAFGP500μg/mLを添加したものを，それぞれ浸漬保存液として使用した．4℃，24時間の低温単純浸漬保存の後，Langendorff装置による再...

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Material Type: 文書・図像類
Title: 微弱電磁場と不凍糖タンパクを用いた低温心保存法
Author/Editor: 鷹合, 真太郎
Takagou, Shintarou
Author Heading: 鷹合, 真太郎
Takagou, Shintarou
Publication Date: 2021-04-28
Publication Date (W3CDTF): 2021-04-28
Alternative Title: Heart Cryopreservation using Antifreeze Glycoprotein in a Variable Magnetic Field
Periodical title: 令和2(2020)年度科学研究費補助金若手研究研究成果報告書 = 2020 Fiscal Year Final Research Report
No. or year of volume/issue: 2019-04-01 - 2021-03-31
Volume: 2019-04-01 - 2021-03-31
Pages: 5p.-
Text Language Code: jpn
Subject Heading: 不凍糖タンパク
低温心保存法
再灌流実験
Target Audience: 一般
Note (General): 金沢大学附属病院心臓血管外科
ラット心を低温単純浸漬法にて保存実験した．control群（n=6）として保存液のみ，AFGP添加群（n=6）として保存液にAFGP500μg/mLを添加したものを，それぞれ浸漬保存液として使用した．4℃，24時間の低温単純浸漬保存の後，Langendorff装置による再灌流実験を実施し，30分，60分，90分，120分経過時点の心拍数，冠灌流量，心収縮力，心拡張力を測定した．また，保存心の組織水分含有量と組織ATP含有量を測定し，それぞれの測定項目について2群間の結果を比較検討した．多くの項目において，AFGP添加群の保存心が運動機能的・代謝機能的に良い状態であったことが示された．
Surgically removed control rat hearts (n = 6) were immersed in UW solution while AFGP-treated hearts (AFGP group) (n = 6) were immersed in UW solution containing 500 μg/mL AFGP. After static hypothermic preservation, a Langendorff apparatus was used to reperfuse the coronary arteries. After 30, 60, 90, and 120 min the heart rate (HR), coronary flow (CF), cardiac contractile force (max dP/dt), and cardiac diastolic force (min dP/dt) were measured. Tissue water content (TWC) and tissue adenosine triphosphate (ATP) levels in the reperfused preserved hearts were also assessed. All parameters were compared between the control and AFGP groups.The higher HR, CF, max dP/dt, min dP/dt, and tissue ATP levels in the AFGP than control hearts suggested that AFGP conferred superior hemodynamic and metabolic functions. Thus, AFGP might be a useful additive for the static/nonfreezing hypothermic preservation of hearts.
研究課題/領域番号:19K18174, 研究期間(年度):2019-04-01 - 2021-03-31
出典：「微弱電磁場と不凍糖タンパクを用いた低温心保存法」研究成果報告書　課題番号19K18174（KAKEN：科学研究費助成事業データベース（国立情報学研究所））（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-19K18174/19K18174seika/）を加工して作成
DOI: 10.24517/00057812
Access Restrictions: 限定公開
Related Material: https://kaken.nii.ac.jp/search/?qm=60619790
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K18174/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-19K18174/19K18174seika/
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 金沢大学 : 金沢大学学術情報リポジトリKURA