飼養環境下の牛群におけるゼアラレノン浸潤動態の解明 : 特に繁殖性との関連性の検証

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Author: 高木, 光博

Publisher: 鹿児島大学

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Note (General)：: 2008-2010年度科学研究費補助金(基盤研究(C))研究成果報告書課題番号：20580355　研究代表者：高木光博 (鹿児島大学農学部准教授)ゼアラレノン（ZEN）はFusarium属真菌により産生されるエストロジェン類似作用を持つマイコトキシン（MT）の1 種であり、ZENに汚染された飼料を...

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Material Type: 文書・図像類
Title: 飼養環境下の牛群におけるゼアラレノン浸潤動態の解明 : 特に繁殖性との関連性の検証
Author/Editor: 高木, 光博
Author Heading: 高木, 光博タカギ, ミツヒロ
Publication, Distribution, etc.: 鹿児島大学
Alternative Title: Monitoring of natural zearalenone contamination in feeds of cattle herds, with special focus on the effect on reproductive efficacy
Text Language Code: jpn
Alias of Author: 髙木, 光博
Subject Heading: ウシ
ブタ
マイコトキシン
ゼアラレノン
尿
卵胞液
Target Audience: 一般
Note (General): 2008-2010年度科学研究費補助金(基盤研究(C))研究成果報告書課題番号：20580355　研究代表者：高木光博 (鹿児島大学農学部准教授)
ゼアラレノン（ZEN）はFusarium属真菌により産生されるエストロジェン類似作用を持つマイコトキシン（MT）の1 種であり、ZENに汚染された飼料を家畜が摂取すると、消化管から吸収されて一部が代謝された後、その代謝物とともに尿中に排出される。これまでに、尿中ZEN濃度をモニターすることで、牛群における給与飼料からのZEN摂取状況の把握が可能となり、ウシ生産現場における比較的簡易な日常のモニタリング法として有用であることを初めて実証するとともに、肥育牛群で特に高い尿中ZEN濃度を示す群が存在したことから，生産現場においては，今後飼料中MT濃度を含めた継続的なMTのモニタリングが必要であることを報告した。また、尿中ZEN濃度をモニターすることで、飼料へのMT吸着剤添加効果の客観的評価が可能となること、およびMT吸着剤の添加方法がその効果に影響を与える可能性を示唆した。さらに、ウシおよびブタ卵胞液からZENおよびその代謝物を検出し、経口摂取後腸管から吸収されたZENが血流を介して卵胞液中へ侵入することを初めて確認し、卵胞発育や卵子成熟への影響も起こりうる可能性を示した。また血中エストロジェン濃度と同等レベルのZENを含む卵胞液サンプルも存在したことから、内分泌撹乱物質として繁殖性に影響を与える可能性も示唆した。
The aims of the first study were (1) to investigate the efficacy of measuring bovine urinary zearalenone (ZEN) concentrations by using a commercially available ELISA method in cattle kept under different feeding conditions to monitor the natural contamination of feeds at the farm level, and (2) to investigate the effects of supplementation of a mycotoxin adsorbent (MA) product in the feed based on urinary ZEN concentration. The findings indicate the usefulness of measuring concentrations of urinary ZEN and its metabolites not only for monitoring the natural ZEN contamination of cattle feed at the farm level but also for in vivo evaluation of MA function after supplementing feeds with MA. Zearalenone (ZEN) and its metabolites are important nonsteroidal estrogenic mycotoxins that cause reproductive disorders in domestic animals, especially pigs. The aim of the second study were to simultaneously detect ZEN and its metabolites α-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) in porcine follicular fluid (FF) by liquid chromatography-tandem mass spectrometry. ZEN and α-ZOL, but not β-ZOL, were detected in all pooled FF samples collected from coexisting follicles (diameter >≥ 6 mm) within 10 ovaries. Furthermore, ZEN and α-ZOL were detected in samples pretreated with β-glucuronidase/arylsulfatase, but not in those left untreated, suggesting that the FF samples contained glucuronide-conjugated forms of the mycotoxins that may be less harmful to porcine oocytes due to glucuronidation affecting the receptor binding. Nonetheless, the effects of the glucuronide-conjugated forms should be studied, both in vitro and in vivo. Additionally, the influence of acute exposure to zearalenone (ZEN) on porcine oocyte maturation, fertilization, or sperm penetration ability during both in vitro maturation and fertilization was evaluated. First, oocytes were cultured in ZEN-containing (0-1000 μg/l) maturation medium and then fertilized. Second, oocytes maturing in vitro without ZEN were fertilized in ZEN-containing fertilization medium. The maturation rates of oocytes and penetration ability of sperm decreased significantly in the presence of 1000 μg/l of ZEN. However, neither increases in the rates of degeneration and DNA fragmentation of oocytes nor reductions in normal and polyspermic fertilization were observed. ZEN did not affect the sperm penetration rates, however, 1000 μg/l ZEN had positive effects on normal and polyspermic fertilization rates. Therefore, it can be suggested that an acute exposure of porcine oocytes during maturation, and oocytes and sperm during fertilization to ZEN up to 1000 μg/l may not affect the fertility of the oocytes.
Format (IMT): application/pdf
Source: 20580355seika.pdf （fulltext）
https://ir.kagoshima-u.ac.jp/record/695/files/20580355seika.pdf
Access Restrictions: 限定公開
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ