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Bibliographic Record
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- Material Type
- 文書・図像類
- Author/Editor
- 西, 順一郎
- Author Heading
- Publication, Distribution, etc.
- Alternative Title
- Molecular-epidemiological study of an emerging pathogen enteroaggregative Eschelichia coli and development of rapid diagnosis using immunochromatography
- Text Language Code
- jpn
- Target Audience
- 一般
- Note (General)
- 2008-2010年度科学研究費補助金(基盤研究(C))研究成果報告書 課題番号：20591282 研究代表者：西順一郎 (鹿児島大学医学部・歯学部附属病院講師)転写調節因子AggRを保有する腸管凝集性大腸菌Enteroaggregative Esherichia coli(EAEC)の検出頻度は、下痢症患児由来大腸菌2,417株中76株(3.1%)であった。菌体表層抗原Aapの検出頻度はEAECでは81.6%と高く、アミノ酸配列はよく保存されていた。イムノクロマト法によるEAEC検出に利用するために、Aapのリコンビナント蛋白を用いて抗Aap抗体を作成し、EAECへの反応性を確認できた。We demonstrated that the incidence of typical enteroaggregative Esherichia coli (EAEC) among E. coli strains isolated from diarrheal children was 3.1% (76/2,417). The incidence of suface-layer protein Aap was 81.6% among typical EAEC strains, and its amino-acid sequences were well conserved. We successfully prepared anti-Aap antibodies using recombinant Aap protein, which can be applied for a rapid immunochromatographic detection system of EAEC.