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- Material Type
- 文書・図像類
- Author/Editor
- 小澤, 真
- Author Heading
- Publication, Distribution, etc.
- Alternative Title
- Generation of transgenic mouse for analysis of in vivo dynamics of influenza virus infection
- Text Language Code
- jpn
- Subject Heading
- Target Audience
- 一般
- Note (General)
- 2012-2013年度科学研究費助成事業（挑戦的萌芽研究）研究成果報告書 課題番号：24658245 研究代表者：小澤真（鹿児島大学・獣医学部・准教授）GFPをコードしたインフルエンザ遺伝子用組換えRNAを発現する遺伝子組換えマウスを作出するため、プラスミドを構築した。プラスミド導入培養細胞へ野生型ウイルスを感染させると、GFPが発現した。プラスミド転写領域のDNAをマウス受精卵に導入し、導入遺伝子陽性マウス3系統を得た。各系統のマウス個体ならびに尻尾由来初代培養細胞に野生型ウイルスを感染させたが、GFPは発現しなかった。本研究の完遂には、プラスミド転写領域の改変が必要と考えられた。To generate transgenic mouse ubiquitously expressing a recombinant influenza virus -like RNA encoding GFP gene, we constructed a plasmid for the expression of the recombinant RNA. Cultured cells pre-transfected with the constructed plasmid and subsequently infected with wild-type influenza viruses expressed GFP specifically in virus-infected cells. By injecting the transcriptional region fragment of the plasmid into mouse fertilized eggs, we obtained three mouse lines whose germlines were positive for the injected genes. The animals or their tail-derived primary cells, however, did not express GFP even after infection with wild-type influenza viruses. These results imply that the transcriptional region of the plasmid should be modified to complete this study.