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- Material Type
- 文書・図像類
- Author/Editor
- 村越, 道生
- Author Heading
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- Alternative Title
- Study of molecular mechanisms of structual change of prestin using a nvel atomic force microscope combined with a patch clamp system
- Text Language Code
- jpn
- Target Audience
- 一般
- Note (General)
- 2012-2013年度科学研究費助成事業（若手研究（B））研究成果報告書 課題番号：24791739 研究代表者：村越道生（鹿児島大学・理工学研究科・准教授）細胞膜電位コントロールデバイスを開発し、これを用いてプレスチンを発現させた培養細胞の電気特性計測及び単離細胞膜表面の微細構造観察を試みた。その結果。デバイス基盤の微小孔（直径約2um）上に、細胞をポジショニングすることに成功し、細胞膜表面の詳細構造の可視化にも成功した。さらに、デバイス基盤の微小孔上に、人工脂質膜を作成することを試み、脂質膜形成後にギガオームシールが形成されることが確認された。このことは、当該デバイスで脂質膜中に再構成したタンパク質の変形挙動を可視化できる可能性を示唆している。今後、得られた知見を基に、プレスチンの変形挙動の可視化に取り組む。A cell membrane potential control device was developed. Using this device, the microstructure and electrophysiological properties of prestin-expressing culture cells were investigated. A cell was successfully positioned on a micropore of the device (about 2 um in diameter) and the microstructure of the cell surface was visualized. In addition, an artificial lipid bilayer was created on the micropore, resulting in the formation of a gigaohm seal. These results suggest that it may be possible to visualize protein reconstituted in the lipid bilayer by using this device. Based on these findings, further research to visualize the conformational change of prestin will be performed.