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- Material Type
- 文書・図像類
- Author/Editor
- 長谷川, 大子
- Author Heading
- Publication, Distribution, etc.
- Alternative Title
- Enrichment of the tooth constitution cell derived from human iPS cells using an original technique
- Text Language Code
- jpn
- Subject Heading
- Target Audience
- 一般
- Note (General)
- 2011-2013年度科学研究費助成事業（基盤研究（C））研究成果報告書 課題番号：23593038 研究代表者：長谷川大子（鹿児島大学・医歯（薬）学総合研究科・客員研究員）pT-ARIP導入iPS細胞をヌードマウスに移植し、生じた奇形腫を初代培養し、歯関連細胞の出現を蛍光発現で検索した結果、蛍光を発現する細胞を観察することができなかった。おそらくin vivoで奇形腫が形成される過程で導入された遺伝子にはサイレンシングなどの遺伝子発現抑制がかかっていた可能性がある。そこで、遺伝子導入前のiPS細胞由来の奇形腫から得た初代培養細胞について、RT-PCR解析を行った。その結果、OCT3/4、NANOG、NESTIN、OC（osteocalcin）、DMP、DSPP、BGPの発現が認められた。また、免疫組織学的解析を行ったところ、AMELXの存在を確認した。attempted to detect cells responsible for tooth development based on the expression of the fluorescent gene; however, the cells were not detected.. In the process in which a teratoma was formed, repression of gene expression likes gene silencing may have started the introduced gene. Hence, RT-PCR analysis of the primary teratoma cell culture obtained from iPS cells before transgenesis was conducted. The expression of OCT3/4, NANOG, NESTIN, OC (osteocalcin), DMP, DSPP, and BGP was observed. Moreover, the existence of AMELX was confirmed by immunohistological analysis.