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- Material Type
- 文書・図像類
- Author/Editor
- 西, 順一郎
- Author Heading
- Publication, Distribution, etc.
- Alternative Title
- Pan-genome analysis of virullent and antibiotics-resistan genes among Escherichia coli isolated from diarrheal children
- Text Language Code
- jpn
- Target Audience
- 一般
- Note (General)
- 2011-2013年度科学研究費助成事業（基盤研究（C））研究成果報告書 課題番号：23591574 研究代表者：西順一郎（鹿児島大学・医歯（薬）学総合研究科・教授）2001年～10年の小児下痢症患児由来大腸菌において、腸管凝集性大腸菌（EAEC）はESBL遺伝子を有意に高い頻度で保有していた（20.8%, 31/149 vs. 1.1%, 64/6067; OR 24.6）。ESBL産生EAECのESBL遺伝子型はCTX-M-14、74.2%がO25:H4:ST131、B2群（腸管外病原性大腸菌）に属し、これまでのEAECと明らかに異なる系統であった。以上より、O25:H4:ST131がCTX-M-14とEAEC病原プラスミドを獲得し、小児間で伝播したことが示唆された。EAECはその凝集性から薬剤耐性遺伝子のリザーバーとなる可能性が示唆された。A total of 6,216 E. coli strains from diarrheal children from 2001 through 2010 in our district were examined for serotyping, ESBL genotyping, and virulence genes including aggR by PCR. In addition, MLSA of ESBL-producing EAEC strains were performed. The incidence of ESBL genes in aggR-positive EAEC strains (20.8%, 31/149) was significantly higher than in other E. coli strains (1.1%, 64/6067) (OR 24.6, 95% Cl 15.5-39.3). ESBL genes were not detected in other pathotypes of diarrheagenic E. coli. All 31 ESBL-producing EAEC strains possessed CTX-M-14, most of which were found to be 025:H4:ST131. EAEC 0111 and 0126 strains mainly isolated since 1997 through 2010 never harbored ESBL genes, while the EAEC 025:H4:ST 131 clone producing CTX-M-14 was detected in 2003 for the first time. These findings suggest that the 025:H4:ST131 clone horizontally acquired CTX-M-14 and EAEC virulence genes in our district. EAEC may play a role of reservoir of ESBL genes.