硫化水素によるクラウン系ミニブタ腎虚血再灌流障害抑制効果

• Material Type: 博士論文
• Title: 硫化水素によるクラウン系ミニブタ腎虚血再灌流障害抑制効果
• Author/Editor: 関島, 光裕
• Author Heading: 関島, 光裕 セキジマ, ミツヒロ
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2017
• Publication Date (W3CDTF): 2017
• Alternative Title: Hydrogen Sulfide Prevents Renal Ischemia-Reperfusion Injury in CLAWN Miniature Swine
• Periodical title: Journal of Surgical Research
• No. or year of volume/issue: 219
• Volume: 219
• Pages: 165-172
• Degree grantor/type: 鹿児島大学
• Date Granted: 2018-04-26
• Dissertation Number: 甲総研第460号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: Hydrogen sulfide; Ischemia-reperfusion injury; Large animal model; Proinflammatory cytokines
• Target Audience: 一般
• Note (General): 博士論文全文, 最終試験結果の要旨, 論文審査の要旨, 博士論文要旨; Background: Hydrogen sulfide (H2S) has recently been reported to demonstrate both antiinflammatory and cytoprotective effects; however, its efficacy has not been well documented in large animal models. In this study, we examined whether the administration of H2S offers cytoprotective effects on renal ischemia-reperfusion injury (IRI) in a preclinical miniature swine model. Methods: Major histocompatibility complex-inbred, CLAWN miniature swine (n=9) underwent a right nephrectomy, followed by induction of a 120-min period of warm ischemia via placement of clamps on the left renal artery and vein. Group 1 (n=3) underwent renal ischemia without H2S administration. Groups 2 (n=3) and 3 (n=3) received Na2S (prodrug of H2S) 10 min before reperfusion of the ischemic kidneys followed by a 30-min of Na2S postreperfusion intravenously (group 2) or selective administration of Na2S via the left renalartery(group 3).IRI was assessed by kidney biopsies, levels of inflammatory cytokines in sera and kidney tissue. Results: Animals in group 1 had significantly higher serum creatinine levels compared with animals in groups 2 and 3 (P<0.01). Histology showed severe tubular damage with TUNEL-positive cells in group 1 on postoperative day 2 compared with mild damage in group 2 and minimal damage in group 3. Further more, levels of inflammatory cytokines in both serum (interleukin-6 [IL-6], tumor necrosis factor-α, and high-mobility group box 1) and renal tissue (IL-1 and IL-6) in group 3 were markedly lower than in group 2, suggesting beneficial effects of selective Na2S administration. Conclusions: Na2S administration, especially via an organ selective approach, appears to potentially offer cytoprotective and antiinflammatory effects following rena lIRI.
• Format (IMT): application/pdf
• Source: Diss_関島_光裕_ISK460_2018 （fulltext）; https://ir.kagoshima-u.ac.jp/record/14404/files/Diss_%E9%96%A2%E5%B3%B6_%E5%85%89%E8%A3%95_ISK460_2018.pdf
• Access Restrictions: インターネット公開
• Rights (production): © 2017 Elsevier Inc. All rights reserved.
• Related Material: Journal of Surgical Research; https://www.sciencedirect.com/journal/journal-of-surgical-research
• Is Version Of (URI): URI; https://doi.org/10.1016/j.jss.2017.05.123
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ