ホスホリルコリン経皮投与における全身および粘膜面にあたえる影響に関するマウスを用いた検討

• Material Type: 博士論文
• Title: ホスホリルコリン経皮投与における全身および粘膜面にあたえる影響に関するマウスを用いた検討
• Author/Editor: 永野, 広海
• Author Heading: 永野, 広海 ナガノ, ヒロミ
• Publication, Distribution, etc.: 鹿児島大学
• Publication Date: 2015
• Publication Date (W3CDTF): 2015
• Alternative Title: Transcutaneous immunization with phosphorylcholine induces antigen-specific mucosal and systemic immune responses in BALB/cmice.
• Periodical title: Auris Nasus Larynx
• No. or year of volume/issue: 42
• Volume: 42
• Pages: 478-482
• Degree grantor/type: 鹿児島大学
• Date Granted: 2018-04-04
• Dissertation Number: 甲総研第463号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: Transcutaneous immunization; Phosphorylcholine; Saliva; Secretory IgA
• Target Audience: 一般
• Note (General): 博士論文全文, 最終試験結果の要旨, 論文審査の要旨, 博士論文要旨; Objective: Transcutaneous immunization (TCI) is a novel route of vaccination through application of a topical vaccine antigen on the skin. Phosphorylcholine (PC) is a structural component of a variety of pathogens and anti-PC immune responses protect mice against invasive bacterial diseases. The purpose of the study was to examine the effect of TCI using PC in BALB/c mice. Methods: TCI was performed in BALB/c mice using PC–keyhole limpet hemocyanin (KLH) plus cholera toxin (CT). Immunogenicity was evaluated by measuring PC-specific IgG and specific IgG1, IgG2a, IgM, IgA, and secretory IgA antibodies by ELISA. The concentrations of IL-4, IL-5, IL-10, IL-12, IL-13 and IFN-γ were also measured using ELISA for mouse. Results: Six months after immunization, IgG after TCI using PC plus CT was significantly higher than in controls, but this was not found for IgA. In saliva, secretory IgA antibodies decreased with a peak level at 2–3 months. IgG1 was significantly higher than IgG2 after TCI. Production of IL-4 from CD4+ cells was significantly higher after TCI than in controls, whereas production of IFN-γ, IL-5, IL-12 and IL-13 was not detected in either group. Conclusion: These results suggest that TCI using PC plus CT with BALB/c mice is a simple approach for induction of systemic and mucosal immune responses that are shifted in the Th-2 direction.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © 2015 Elsevier Ireland Ltd. All rights reserved.
• Related Material: URI; https://www.journals.elsevier.com/auris-nasus-larynx
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Nagano_Hiromi_isk_463_2018 other

Comments_Nagano_Hiromi_isk_463_2018 other

Diss 永野 広海 2018年 fulltext

4 Abstract__永野 広海_4510810051_2018. abstract