脳梗塞発症前運動は、ラットの局所脳虚血後、内在性14-3-3γの増強を介して脳損傷および神経細胞のアポトーシスを減少させる

• Material Type: 博士論文
• Title: 脳梗塞発症前運動は、ラットの局所脳虚血後、内在性14-3-3γの増強を介して脳損傷および神経細胞のアポトーシスを減少させる
• Author/Editor: 大塚, 章太郎
• Author Heading: 大塚, 章太郎 オオツカ, ショウタロウ
• Publication, Distribution, etc.: Springer
• Publication Date: 2019
• Publication Date (W3CDTF): 2019
• Alternative Title: Preconditioning exercise reduces brain damage and neuronal apoptosis through enhanced endogenous 14-3-3γ after focal brain ischemia in rats
• Periodical title: Brain Structure and Function
• No. or year of volume/issue: 224 2
• Volume: 224
• Issue: 2
• Pages: 727-738
• Degree grantor/type: 鹿児島大学
• Date Granted: 2019-03-25
• Dissertation Number: 甲保研第13号
• Degree Type: 博士（保健学）; Doctor of Philosophy in Health Science
• Text Language Code: eng
• Subject Heading: 14-3-3γ; HIF-1α; physical activity; ischemic tolerance; intrinsic factor
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; 14-3-3γ is an important early ischemia-inducible protective factor against ischemic cell death in cerebral cortical neurons. We investigated the anti-apoptosis mechanism of enhanced 14-3-3γ mediated by preconditioning exercise-induced brain ischemic tolerance after stroke. Rats were assigned to 4 groups: exercise and ischemia (Ex group), ischemia and no exercise (No-Ex group), exercise and no ischemia (Ex-only group), and no exercise and ischemia (control group). Rats were trained on a treadmill for 5 days a week for 3 weeks (running speed, 25 m/min; running duration, 30 min/day). After the exercise program, stroke was induced by left middle cerebral artery occlusion. The infarct volume, neurological deficits, and motor function, as well as expression levels of hypoxiainduced factor-1α (HIF-1α), 14-3-3γ, P2X7 receptors, p-β-catenin Ser37, Bax, and caspase 3 were evaluated by immunohistochemistry and western blotting. The expression of HIF-1α and 14-3-3γ significantly increased in neurons and astrocytes in the Ex-only group. HIF-1α was co-expressed with P2X7 receptor- and GFAP-positive astrocytes. After stroke, the Ex group had significantly reduced brain infarction. HIF-1α and 14-3-3γ significantly increased in the Ex group compared to the No-Ex group. In addition, p-β-catenin Ser37 significantly increased following elevated 14-3-3γ; in contrast, Bax and caspase 3 were significantly reduced in the Ex group. Our findings suggest that preconditioning exercise prior to ischemia induces neuron- and astrocyte-mediated brain ischemic tolerance through increased expression of HIF-1α and 14-3-3γ, which are intrinsic protective factors; the upregulated 14-3-3γ induced by preconditioning exercise reduces ischemic neuronal cell death through the 14-3-3γ/p-β-catenin Ser37/Bax/caspase 3 anti-apoptotic pathway.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © Springer-Verlag GmbH Germany, part of Springer Nature 2018
• Related Material (DOI): 10.1007/s00429-018-1800-4
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Otsuka_Shotaro_hok13_2019 other

Comments_Otsuka_Shotaro_hok13_2019 other

Diss_大塚_章太郎_HOK013_2018 fulltext

Abstract_大塚_章太郎_4716700015_2018 abstract