ルビスコリン-６はオピオイド受容体を活性化することで骨格筋の糖取込みを促進する

• Material Type: 博士論文
• Title: ルビスコリン-６はオピオイド受容体を活性化することで骨格筋の糖取込みを促進する
• Author/Editor: Kairupan, Timothy Sean
• Author Heading: Kairupan, Timothy Sean
• Publication, Distribution, etc.: Food and Drug Administration
• Publication Date: 2019
• Publication Date (W3CDTF): 2019
• Alternative Title: Rubiscolin-6 activates opioid receptors to enhance glucose uptake in skeletal muscle
• Periodical title: Journal of Food and Drug Analysis
• No. or year of volume/issue: 27 1
• Volume: 27
• Issue: 1
• Pages: 266-274
• Degree grantor/type: 鹿児島大学
• Date Granted: 2019-03-19
• Dissertation Number: 甲総研第495号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: AMPK; Glucose Uptake; GLUT4; Opioid Receptor; Rubiscolin-6
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Rubiscolin-6 is an opioid peptide derived from plant ribulose bisphosphate carboxylase/oxygenase (Rubisco). It has been demonstrated that opioid receptors could control glucose homeostasis in skeletal muscle independent of insulin action. Therefore, Rubiscolin-6 may be involved in the control of glucose metabolism. In the present study, we investigated the effect of rubiscolin-6 on glucose uptake in skeletal muscle. Rubiscolin-6-induced glucose uptake was measured using the fluorescent indicator 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxyglucose (2-NBDG) in L6 and C2C12 cell lines. The protein expressions of glucose transporter 4 (GLUT4) and AMP-activated protein kinase (AMPK) in L6 cells were observed by Western blotting. The in vivo effects of rubiscolin-6 were characterized in streptozotocin (STZ)-induced diabetic rats. Rubiscolin-6 induced a concentration-dependent increase in glucose uptake levels. The increase of phospho-AMPK (pAMPK) and GLUT4 expressions were also observed in L6 and C2C12 cells. Effects of rubiscolin-6 were blocked by opioid receptor antagonists and/or associated signals inhibitors. Moreover, Rubiscolin-6 produced a dose-dependent reduction of blood glucose and increased GLUT4 expression in STZ-induced diabetic rats. In conclusion, rubiscolin-6 increases glucose uptake, potentially via an activation of AMPK to enhance GLUT4 translocation after binding to opioid receptors in skeletal muscle.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © 2018 Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.
• Related Material: URI; https://www.jfda-online.com/
• Related Material (DOI): 10.1016/j.jfda.2018.06.012
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Timothy_Sean_Kairupan_isk_495 other

Comments_Timothy_Sean_Kairupan_isk_495 other

Diss_Kairupan_Timothy_ISK495_2018 fulltext

Abstract_Kairupan_Timothy_4514800080_2018 abstract