膵管腺癌における分子病態 : Pre-miR-148aのパッセンジャー鎖が遺伝子調節に及ぼす影響。

• Material Type: 博士論文
• Title: 膵管腺癌における分子病態 : Pre-miR-148aのパッセンジャー鎖が遺伝子調節に及ぼす影響。
• Author/Editor: 伊地知, 徹也
• Author Heading: 伊地知, 徹也 イヂチ, テツヤ
• Publication, Distribution, etc.: Japanese Cancer Association
• Publication Date: 2018
• Publication Date (W3CDTF): 2018
• Alternative Title: Molecular pathogenesis of pancreatic ductal adenocarcinoma : impact of passenger strand of pre-miR-148a on gene regulation
• Periodical title: Cancer Science
• No. or year of volume/issue: 109 6
• Volume: 109
• Issue: 6
• Pages: 2013-2026
• Degree grantor/type: 鹿児島大学
• Date Granted: 2019-03-25
• Dissertation Number: 甲総研第502号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: cancer genome/genetics; characteristics and pathology of human cancer; information; invasion and metastasis; oncogenes and tumor-suppressor genes
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; We previously used RNA sequencing to establish the microRNA (miRNA) expression signature of pancreatic ductal adenocarcinoma (PDAC). We found that both strands of pre-miR-148a (miR-148a-5p: the passenger strand and miR-148a-3p: the guide strand) were downregulated in cancer tissues. Ectopic expression of miR-148a-5p and miR-148a-3p significantly inhibited cancer cell migration and invasion, indicating that both strands of pre-miR-148a had tumor-suppressive roles in PDAC cells. In silico database and genome-wide gene expression analyses identified a total of 15 genes that were putative targets regulated by these miRNAs. High expression of miR-148a-5p targets (PHLDA2, LPCAT2 and AP1S3) and miR-148a-3p targets (SMA, ENDOD1 and UHMK1) was associated with poor prognosis of patients with PDAC. Moreover, knockdown of PHLDA2 expression inhibited cancer cell aggressiveness, suggesting PHLDA2 acted as an oncogene in PDAC cells. Involvement of the passenger strand of pre-miR-148a (miR-148-5p) is a new concept in cancer research. Novel approaches that identify tumor-suppressive miRNA regulatory networks in lethal PDAC might provide new prognostic markers and therapeutic targets for this disease.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material: URI; https://onlinelibrary.wiley.com/journal/13497006#pane-01cbe741-499a-4611-874e-1061f1f4679e01
• Related Material (DOI): 10.1111/cas.13610
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Ijichi_Tetsuya_isk_502 other

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Diss_伊地知_徹也_ISK502_2018 fulltext

Abstract_伊地知_徹也_4515810026_2018 abstract