ヒト乳癌細胞株SK-BR-3において、PCP4/PEP19はCYP19A1 promoter I.1を介してaromatase遺伝子発現を亢進する。

• Material Type: 博士論文
• Title: ヒト乳癌細胞株SK-BR-3において、PCP4/PEP19はCYP19A1 promoter I.1を介してaromatase遺伝子発現を亢進する。
• Author/Editor: 本庄, 希江
• Author Heading: 本庄, 希江 ホンジョウ, キエ
• Publication, Distribution, etc.: Oncotarget
• Publication Date: 2018
• Publication Date (W3CDTF): 2018
• Alternative Title: PCP4/PEP19 Upregulates Aromatase Gene Expression via CYP19A1 Promoter I.1 in Human Breast Cancer SK-BR-3 Cells
• Periodical title: Oncotarget
• No. or year of volume/issue: 9 51
• Volume: 9
• Issue: 51
• Pages: 29619-29633
• Degree grantor/type: 鹿児島大学
• Date Granted: 2019-03-25
• Dissertation Number: 甲総研第506号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: PCP4/PEP19; breast cancer; aromatase; intratumoral heterogeneity
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; The Purkinje cell protein 4/peptide 19 (PCP4/PEP19) is a novel breast cancer cell expressing peptide, originally found in the neural cells as an anti-apoptotic factor, could inhibit cell apoptosis and enhance cell migration and invasion in human breast cancer cell lines. The expression of PCP4/PEP19 is induced by estrogens in estrogen receptor-positive (ER+) MCF-7 cells but also highly expressed in ER- SK-BR-3 cells. In this study, we investigated the effects of PCP4/PEP19 on aromatase gene expression in MCF-7 and SK-BR-3 human breast cancer cells. In SK-BR-3 cells but not in MCF-7 cells, PCP4/PEP19 knockdown by siRNA silencing decreased the aromatase expression in gene transcriptional level. When PCP4/PEP19 was overexpressed by CMV promoterdriven PCP4/PEP19 expressing plasmid transfection, aromatase gene transcription increased in SK-BR-3 cells. This aromatase gene transcription is mainly mediated through promoter region PI.1, which is usually active in the placental tissue but not in the breast cancer tissue. These results indicate a new function of PCP4/PEP19 that would enhance aromatase gene upregulation to supply estrogens in heterogeneous cancer microenvironment.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material: URI; http://www.oncotarget.com/
• Related Material (DOI): 10.18632/oncotarget.25651
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Honjyo_Kie_isk_506 other

Comments_Honjyo_Kie_isk_506 other

Diss_本庄_希江_ISK506_2018 fulltext

Abstract_本庄_希江_4515810151_2018 abstract