肺炎球菌と無萊膜インフルエンザ菌の上皮細胞接着におけるホスホリルコリンの役割

• Material Type: 博士論文
• Title: 肺炎球菌と無萊膜インフルエンザ菌の上皮細胞接着におけるホスホリルコリンの役割
• Author/Editor: 井内, 寛之
• Author Heading: 井内, 寛之 イウチ, ヒロユキ
• Publication, Distribution, etc.: elsevier
• Publication Date: 2018
• Publication Date (W3CDTF): 2018
• Alternative Title: Role of phospharylcholine in Streptococcus pneumoniae and nontypeable Haemophilus influenzae adherence to epithelial cells
• Periodical title: Auris Nasus Larynx
• Degree grantor/type: 鹿児島大学
• Date Granted: 2019-04-25
• Dissertation Number: 甲総研第509号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: Phosphorylcholine; Streptococcus pneumoniae; Nontypeable Haemophilus influenza; PAF-R
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Objective: Phosphorylcholine (PC) is a structural component of Streptococcus pneumoniae (Spn) and nontypeable Haemophilus influenzae (NTHi), and is known to be associated with adherence through the platelet activating factor receptor (PAF-R). Furthermore, high PC expression is considered to be involved in Spn and NTHi virulence. In this study, we examined the influence of PC expression on the adherence of Spn and NTHi to epithelial cells in order to clarify the potential effectiveness of a vaccine targeting PC. Methods: Twenty-seven strains of Spn and twenty-two strains of NTHi were used, cultured overnight, and PC expression was evaluated by fluorescence activated cell sorting; the strains were divided into two groups: PC low expression (PC-low) and PC high expression (PC-high) groups. Bacterial adherence was then examined using Detroit 562 cells and BALB/c mice. Bacterial invasion was then examined in Detroit 562 cells. Results: The adherence of Spn and NTHi and invasion of NTHi in the PC-high group was significantly reduced by pretreatment with a monoclonal anti-PC antibody (TEPC-15), PAF-R antagonist (ABT-491), and PC-keyhole limpet hemocyanin (PC-KLH). However, such findings were not observed in the PC-low group. Conclusion: The present study suggests that PC is involved in the mucosal adhesion of Spn and NTHi, and the mucosal invasion of NTHi with PC-high strains, but not PC-low strains. These results suggest that a PC-targeting mucosal vaccine only affects PC-high Spn and NTHi strains and does not disturb commensal bacterial flora in the upper respiratory tract, which comprises nonpathogenic PClow bacteria.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material: URI; https://www.journals.elsevier.com/auris-nasus-larynx/
• Related Material (DOI): 10.1016/j.anl.2018.11.003
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Iuchi_Hiroyuki_isk_509 other

Comments_Iuchi_Hiroyuki_isk_509 other

Diss_井内_寛之_ISK509_2019 fulltext

Abstract_井内_寛之_4514810040_2018 abstract