Synergistic effect of arsenic trioxide, vismodegib and temozolomide on glioblastoma

• Material Type: 博士論文
• Title: Synergistic effect of arsenic trioxide, vismodegib and temozolomide on glioblastoma
• Author/Editor: Bureta, Costansia Anselim
• Author Heading: Bureta, Costansia Anselim
• Publication, Distribution, etc.: Spandidos Publications
• Degree grantor/type: 鹿児島大学
• Date Granted: 2020-03-25
• Dissertation Number: 甲総研第553号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: glioblastoma; arsenic trioxide; vismodegib; temozolomide; synergism; Hedgehog inhibitor
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; The treatment of glioblastoma is a critical health issue, owing to its resistance to chemotherapy. The current standard of treatment is surgical resection, followed by adjuvant radiotherapy and temozolomide treatment. Long‑term local treatment of glioblastoma is rarely achieved and the majority of the patients undergo relapse. Resistance to temozolomide emerges from numerous signalling pathways that are altered in glioblastoma, including the Hedgehog signalling pathway. Hence, further research is required to identify effective treatment modalities. We investigated the effect of vismodegib, arsenic trioxide and temozolomide on glioblastoma in vitro and in vivo to apply our findings to the clinical setting. WST‑1 assay revealed that glioblastoma proliferation was inhibited following treatment with these drugs either in single or in combination; this synergistic effect was confirmed by CalcuSyn software. Western blot analysis revealed an increase in the expression of cleaved caspase‑3 and γH2AX. Furthermore, there was marked inhibition and decreased tumour growth in mice that received combination therapy, unlike those that received single agent or vehicle treatment. Our results revealed that the combination of arsenic trioxide/vismodegib and temozolomide may be an attractive therapeutic method for the treatment of glioblastoma. Costansia Bureta, Yoshinobu Saitoh, Hiroto Tokumoto, Hiromi Sasaki, Shingo Maeda, Satoshi Nagano, Setsuro Komiya, Noboru Taniguchi, Takao Setoguchi Synergistic effect of arsenic trioxide, vismodegib and temozolomide on glioblastoma Oncology Reports 2019 41(6) 3404-3412 https://doi.org/10.3892/or.2019.7100
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Costansia_Anselim_Bureta_isk_553 other

Comments_Costansia_Anselim_Bureta_isk_553 other

Diss_Bureta_Costansia_Anselim_4516810245_2019 fulltext

Abstract_Bureta_Costansia_Anselim_4516810245_2019 abstract