内視鏡治療後胃潰瘍に対するヘキサノイル化アルカリ処理ゼラチンシート(HAG)の被覆は、炎症と線維化が抑制された適切な潰瘍治癒を誘導する

• Material Type: 博士論文
• Title: 内視鏡治療後胃潰瘍に対するヘキサノイル化アルカリ処理ゼラチンシート(HAG)の被覆は、炎症と線維化が抑制された適切な潰瘍治癒を誘導する
• Author/Editor: 前田, 英仁
• Author Heading: 前田, 英仁 マエダ, ヒデヒト
• Publication, Distribution, etc.: Karger
• Alternative Title: Cowering post-endoscopic submucosal dissection ulcers in miniature swine with hexanoy1 (Hx:C6) group-modified alkaline-treated gelatin porous film(HAG) induces proper healing by decreasing inflammation and fibrosis
• Degree grantor/type: 鹿児島大学
• Date Granted: 2020-10-16
• Dissertation Number: 甲総研第578号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: endoscopic submucosal dissection; gastric ulcer; fibrosis
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Background and Aims: Hexanoyl (Hx:C6) group-modified alkaline-treated gelatin porous film (HAG) is a newly developed degradable hydrogel characterized by strong adhesiveness and high affinity for vascular endothelial growth factor (VEGF). The aim of this study was to clarify the effect of HAG sheets on the healing process of post-endoscopic submucosal dissection (ESD) porcine gastric artificial ulcers. Methods: (1) To evaluate the adhesiveness of HAG sheets over time, we performed ESD to create 1 artificial ulcer and covered the lesion with 1 HAG sheet using 1 miniature swine. We observed 2 ulcers by endoscopic and microscopic examinations. (2) To examine the effect of HAG sheets on post-ESD ulcer healing, we performed ESD using 5 miniature swine. The artificial ulcers were covered with HAG sheets, or left uncovered after ESD (day 0), followed by macroscopic and microscopic examinations. On days 7 and 14, we observed 2 ulcers by endoscopic examinations. On day 14, the animals were sacrificed, and histological examination was performed on the 3 stomachs that could be extirpated. Results: (1) On day 7, adhesion of HAG sheets was observed. (2) Gastric ulcer area on day 7 was significantly larger in the covered ulcers than in the non-covered ulcers (p = 0.046). On day 14, although there was no significant difference in ulcer area irrespective of covering (p = 0.357), the covered ulcers tended to repair less fold convergence than non-covered ulcers. The covered ulcer sheets significantly decreased inflammatory cell infiltration (p = 0.011), but significantly increased the abundance of macrophages (p = 0.033), in submucosal layers. Also, the abundance of alpha-smooth muscle actin-positive cells in submucosal layers of the covered ulcers was significantly reduced (p = 0.044), leading to a decrease in collagen accumulation. In addition, fibrosis and atrophy of the muscularis propria were significantly lower for covered ulcers than for non-covered ulcers. Furthermore, microvessels and VEGF-positive cells were significantly more abundant in the submucosal layers of the covered ulcers (p < 0.001 and p = 0.024, respectively). Conclusions: HAG sheets induced post-ESD ulcer healing with less submucosal inflammation and muscularis propria injury and have the potential to decrease excess scarring. Hidehito Maeda, Fumisato Sasaki, Yuko Morinaga, Masayuki Kabayama, Hiromichi Iwaya, Yuga Komaki, Shiho Arima, Yuichiro Nasu, Shiroh Tanoue, Shinichi Hashimoto, Shuji Kanmura, Akihiro Nishiguchi, Tetsushi Taguchi, Akio Ido Covering Post-Endoscopic Submucosal Dissection Ulcers in Miniature Swine with Hexanoyl (Hx:C6) Group-Modified Alkaline-Treated Gelatin Porous Film (HAG) Induces Proper Healing by Decreasing Inflammation and Fibrosis This is the peer-reviewed but unedited manuscript version of the following article: Digestion 2021;102:415–427 (DOI: 10.1159/000509056)]. The final, published version is available at http://www.karger.com/?doi=10.1159/000509056.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Maeda_Hidehito_isk_578 other

Comments_Maeda_Hidehito_isk_578 other

Diss_前田_英仁_ISK578_2020 fulltext

Abstract_前田_英仁_4515800119_2020 abstract