ヒトカテリシジン抗菌性ペプチドLL-37はERK及びAktシグナル伝達経路を介してリンパ管内皮細胞によるリンパ管新生を促進する

• Material Type: 博士論文
• Title: ヒトカテリシジン抗菌性ペプチドLL-37はERK及びAktシグナル伝達経路を介してリンパ管内皮細胞によるリンパ管新生を促進する
• Author/Editor: 柳澤, 嵩大
• Author Heading: 柳澤, 嵩大 ヤナギサワ, タカヒロ
• Publication, Distribution, etc.: Springer
• Alternative Title: Human Cathelicidin Antimicrobial Peptide LL-37 Promotes Lymphangiogenesis in Lymphatic Endothelial Cells through the ERK and Akt Signaling Pathways
• Degree grantor/type: 鹿児島大学
• Date Granted: 2020-11-13
• Dissertation Number: 甲総研第579号
• Degree Type: 博士(歯学); Doctor of Philosophy in Dental Science
• Text Language Code: eng
• Subject Heading: LL-37; Lymphangiogenesis; ERK; Akt; FPRL1
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; LL-37, the only member of the cathelicidin family of cationic antimicrobial peptides in humans has been shown to exhibit a wide variety of biological actions in addition to its antimicrobial activity. However, the lymphangiogenic effect of LL-37 has not been elucidated yet. In this study, we examined the effects of LL-37 on lymphangiogenesis and evaluated the underlying molecular mechanisms. LL-37 treatment significantly increased the migration and tube-like formation of human dermal lymphatic microvascular endothelial cells (HDLECs) and promoted the expression of lymphangiogenic factor in HDLECs. Treatment with LL-37 increased phosphorylation of ERK and Akt proteins in HDLECs, and pretreatment with ERK and Akt inhibitors significantly blocked the LL-37-induced HDLEC migration and tube-like formation. Furthermore, to investigate the involvement of formyl peptide receptor-like 1 (FPRL1) signaling in LL-37-induced lymphangiogenesis, HDLECs were treated with an FPRL1 antagonist. Pretreatment with the FPRL1 antagonist inhibited LL-37-induced phosphorylation of ERK and Akt proteins and attenuated LL-37-induced HDLEC migration and tube-like formation. These data indicated that LL-37 induces lymphangiogenesis in lymphatic endothelial cells via FPRL1, and the activation of the ERK and Akt-dependent signaling pathways. Takahiro Yanagisawa, Masakazu Ishii, Manami Takahashi, Kei Fujishima & Masahiro Nishimura Human cathelicidin antimicrobial peptide LL-37 promotes lymphangiogenesis in lymphatic endothelial cells through the ERK and Akt signaling pathways Molecular Biology Reports volume 47, pages6841–6854 (2020) This is a post-peer-review, pre-copyedit version of an article published in Molecular Biology Reports . The final authenticated version is available online at: https://doi.org/10.1007/s11033-020-05741-8
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Yanagisawa_Takahiro_isk_579 other

Comments_Yanagisawa_Takahiro_isk_579 other

Diss_柳澤_嵩大_ISK579_2020 fulltext

Abstract_柳澤_嵩大_4516810414_2020 abstract