身体小奇形、知的障害と統合失調症を呈する日本人家系におけるポジショナルクローニング法と包括的変異解析による新規KDM2B遺伝子変異の同定

• Material Type: 博士論文
• Title: 身体小奇形、知的障害と統合失調症を呈する日本人家系におけるポジショナルクローニング法と包括的変異解析による新規KDM2B遺伝子変異の同定
• Author/Editor: 横塚, 紗永子
• Author Heading: 横塚, 紗永子 ヨコツカ, サエコ
• Publication, Distribution, etc.: nature
• Alternative Title: Positional cloning and comprehensive mutation analysis identified a novel KDM2B mutation in a Japanege family with minor malformations, intellectual disability, and schizophrenia
• Degree grantor/type: 鹿児島大学
• Date Granted: 2021-03-17
• Dissertation Number: 甲総研第589号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: KDM2B; Positional cloning; Haplotype analysis; Whole-exome sequencing; Histone H3 lysine 4 demethylase; Schizophrenia; Marfan syndrome-like minor physical anomalies
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; The importance of epigenetic control in the development of the central nervous system has recently been attracting attention. Methylation patterns of lysine 4 and lysine 36 in histone H3 (H3K4 and H3K36) in the central nervous system are highly conserved among species. Numerous complications of body malformations and neuropsychiatric disorders are due to abnormal histone H3 methylation modifiers. In this study, we analyzed a Japanese family with a dominant inheritance of symptoms including Marfan syndrome-like minor physical anomalies (MPAs), intellectual disability, and schizophrenia (SCZ). We performed positional cloning for this family using a single nucleotide polymorphism (SNP) array and whole-exome sequencing, which revealed a missense coding strand mutation (rs1555289644, NM_032590.4: c.2173G>A, p.A725T) in exon 15 on the plant homeodomain of the KDM2B gene as a possible cause of the disease in the family. The exome sequencing revealed that within the coding region, only a point mutation in KDM2B was present in the region with the highest logarithm of odds score of 2.41 resulting from whole genome linkage analysis. Haplotype analysis revealed co-segregation with four affected family members (IV-9, III-4, IV-5, and IV-8). Lymphoblastoid cell lines from the proband with this mutation showed approximately halved KDM2B expression in comparison with healthy controls. KDM2B acts as an H3K4 and H3K36 histone demethylase. Our findings suggest that haploinsufficiency of KDM2B in the process of development, like other H3K4 and H3K36 methylation modifiers, may have caused MPAs, intellectual disability, and SCZ in this Japanese family. Saeko Yokotsuka-Ishida, Masayuki Nakamura, Yoko Tomiyasu, Mio Nagai, Yuko Kato, Akiyuki Tomiyasu, Hiromi Umehara, Takehiro Hayashi, Natsuki Sasaki, Shu-ichi Ueno & Akira Sano Positional cloning and comprehensive mutation analysis identified a novel KDM2B mutation in a Japanese family with minor malformations, intellectual disability, and schizophrenia Journal of Human Genetics volume 66, pages597–606 (2021) https://doi.org/10.1038/s10038-020-00889-4
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Yokotsuka_Saeko_isk_589 other

Comments_Yokotsuka_Saeko_isk_589 other

Diss_横塚_紗永子_ISK589_2020 fulltext

Abstract_横塚_紗永子_4515800146_2020 abstract