スニチニプ耐性腎細胞癌に対する新世代mTOR阻害剤Rapalink-1の新規治療効果の可能牲

• Material Type: 博士論文
• Title: スニチニプ耐性腎細胞癌に対する新世代mTOR阻害剤Rapalink-1の新規治療効果の可能牲
• Author/Editor: 黒島, 和樹
• Author Heading: 黒島, 和樹 クロシマ, カズキ
• Publication, Distribution, etc.: Wiley
• Alternative Title: Potential new therapy of Rapalink-1, a new generation mTOR inhibitor, against sunitinib-resistant renal cell carcinoma.
• Degree grantor/type: 鹿児島大学
• Date Granted: 2021-03-17
• Dissertation Number: 甲総研第591号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: mTOR inhibitor; Rapalink-1; renal cell carcinoma; sunitinib resistance; temsirolimus
• Target Audience: 一般
• Note (General): 博士論文全文, 最終試験結果の要旨, 論文審査の要旨; Sunitinib, a multitargeted receptor tyrosine kinase inhibitor including vascular endothelial growth factor, has been widely used as a first-line treatment against metastatic renal cell carcinoma (mRCC). However, mRCC often acquires resistance to sunitinib, rendering it difficult to treat with this agent. Recently, Rapalink-1, a drug that links rapamycin and the mTOR kinase inhibitor MLN0128, has been developed with excellent therapeutic effects against breast cancer cells carrying mTOR resistance mutations. The aim of the present study was to evaluate the in vitro and in vivo therapeutic efficacy of Rapalink-1 against renal cell carcinoma (RCC) compared to temsirolimus, which is commonly used as a small molecule inhibitor of mTOR and is a derivative of rapamycin. In comparison with temsirolimus, Rapalink-1 showed significantly greater effects against proliferation, migration, invasion and cFolony formation in sunitinib-naïve RCC cells. Inhibition was achieved through suppression of the phosphorylation of substrates in the mTOR signal pathway, such as p70S6K, eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) and AKT. In addition, Rapalink-1 had greater tumor suppressive effects than temsirolimus against the sunitinib-resistant 786-o cell line (SU-R 786-o), which we had previously established, as well as 3 additional SU-R cell lines established here. RNA sequencing showed that Rapalink-1 suppressed not only the mTOR signaling pathway but also a part of the MAPK signaling pathway, the ErbB signaling pathway and ABC transporters that were associated with resistance to several drugs. Our study suggests the possibility of a new treatment option for patients with RCC that is either sunitinib-sensitive or sunitinib-resistant. Kazuki Kuroshima, Hirofumi Yoshino, Shunsuke Okamura, Masafumi Tsuruda, Yoichi Osako, Takashi Sakaguchi, Satoshi Sugita, Shuichi Tatarano, Masayuki Nakagawa, Hideki Enokida Potential new therapy of Rapalink-1, a new generation mammalian target of rapamycin inhibitor, against sunitinib-resistant renal cell carcinoma Cancer Science 2020 111(5) p.1607-1618 https://doi.org/10.1111/cas.14395
• Format (IMT): application/pdf
• Source: Diss_黒島_和樹_ISK591_2020 （fulltext）; https://ir.kagoshima-u.ac.jp/record/15836/files/Diss_%E9%BB%92%E5%B3%B6_%E5%92%8C%E6%A8%B9_ISK591_2020.pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ