顎骨骨髄由来間質細胞の in vivo 骨形成能予測マーカーとしての chitinase-3-like protein 1 の有効性

• Material Type: 博士論文
• Title: 顎骨骨髄由来間質細胞の in vivo 骨形成能予測マーカーとしての chitinase-3-like protein 1 の有効性
• Author/Editor: 駒走, 尚大
• Author Heading: 駒走, 尚大 コマバシリ, ナオヒロ
• Publication, Distribution, etc.: Elsevier B.V.
• Alternative Title: Efficacy of chitinase-3-like protein 1 as an in vivo bone formation predictable marker of maxillary/mandibular bone marrow stramal cells
• Degree grantor/type: 鹿児島大学
• Date Granted: 2021-07-15
• Dissertation Number: 甲総研第617号
• Degree Type: 博士(歯学); Doctor of Philosophy in Dental Science
• Text Language Code: eng
• Subject Heading: Jaw bone marrow stromal cells; Bone formation capacity; Chitinase-3-like protein 1; Migration
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Introduction: Maxillary/mandibular bone marrow stromal cells (MBMSCs) are a useful cell source for bone regeneration in the oral and maxillofacial region. To further ensure the clinical application of MBMSCs in bone regenerative therapy, it is important to determine the bone formation capacity of MBMSCs before transplantation. The aim of this study is to identify the molecular marker that determines the in vivo bone formation capacity of MBMSCs. Methods: The cell growth, cell surface antigens, in vitro and in vivo bone formation capacity of MBMSCs were examined. The amount of chitinase-3-like protein 1 (CHI3L1) secreted into the conditioned medium was quantified. The effects of CHI3L1 on the cell growth and osteogenic differentiation potential of MBMSCs and on the cell growth and migration of vascular endothelial cells and fibroblasts were examined. Results: The cell growth, and in vitro and in vivo bone formation capacity of the cells treated with different conditions were observed. MBMSCs that secreted a large amount of CHI3L1 into the conditioned medium tended to have low in vivo bone formation capacity, whereas MBMSCs that secreted a small amount of CHI3L1 had greater in vivo bone formation capacity. CHI3L1 promoted the migration of vascular endothelial cells, and the cell growth and migration of fibroblasts. Conclusion: Our study indicates that the in vitro osteogenic differentiation capacity of MBMSCs and the in vivo bone formation capacities of MBMSCs were not necessarily correlated. The transplantation of high CHI3L1 secretory MBMSCs may suppress bone formation by inducing fibrosis at the site. These results suggest that the CHI3L1 secretion levels from MBMSCs may be used as a predictable marker of bone formation capacity in vivo. Naohiro Komabashiri, Fumio Suehiro, Masakazu Ishii, Masahiro Nishimura Efficacy of chitinase-3-like protein 1 as an in vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells Regenerative Therapy 18 (2021) 38-50 https://doi.org/10.1016/j.reth.2021.03.004
• Format (IMT): application/pdf
• Source: Diss_駒走_尚大_ISK617_2021 （fulltext）; https://ir.kagoshima-u.ac.jp/record/15894/files/Diss_%E9%A7%92%E8%B5%B0_%E5%B0%9A%E5%A4%A7_ISK617_2021.pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ