味蕾におけるⅡ型・Ⅲ型細胞の構成およびNCAMの発現

• Material Type: 博士論文
• Title: 味蕾におけるⅡ型・Ⅲ型細胞の構成およびNCAMの発現
• Author/Editor: 小栁, 江梨子
• Author Heading: 小栁, 江梨子 コヤナギ, エリコ
• Publication, Distribution, etc.: Springer Nature
• Alternative Title: Type II/III cell composition and NCAM expression in taste buds
• Degree grantor/type: 鹿児島大学
• Date Granted: 2021-09-14
• Dissertation Number: 甲総研第620号
• Degree Type: 博士(歯学); Doctor of Philosophy in Dental Science
• Text Language Code: eng
• Subject Heading: Cell-type marker; Taste cell diferentiation; Whole-mount immunohistochemistry; Regional diference; Stem/ progenitor cell
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Taste buds are localized in fungiform (FF), foliate (FL), and circumvallate (CV) papillae on the tongue, and taste buds also occur on the soft palate (SP). Mature elongate cells within taste buds are constantly renewed from stem cells and classified into three cell types, Types I, II, and III. These cell types are generally assumed to reside in respective taste buds in a particular ratio corresponding to taste regions. A variety of cell-type markers were used to analyze taste bud cells. NCAM is the first established marker for Type III cells and is still often used. However, NCAM was examined mainly in the CV, but not sufficiently in other regions. Furthermore, our previous data suggested that NCAM may be transiently expressed in the immature stage of Type II cells. To precisely assess NCAM expression as a Type III cell marker, we first examined Type II and III cell-type markers, IP3R3 and CA4, respectively, and then compared NCAM with them using whole-mount immunohistochemistry. IP3R3 and CA4 were segregated from each other, supporting the reliability of these markers. The ratio between Type II and III cells varied widely among taste buds in the respective regions (Pearson’s r = 0.442 [CV], 0.279 [SP], and − 0.011 [FF]), indicating that Type II and III cells are contained rather independently in respective taste buds. NCAM immunohistochemistry showed that a subset of taste bud cells were NCAM(+)CA4(−). While NCAM(+)CA4(−) cells were IP3R3(−) in the CV, the majority of them were IP3R3(+) in the SP and FF. Eriko Koyanagi-Matsumura, Hirohito Miura, Mitsuru Saito, Shuitsu Harada Type II/III cell composition and NCAM expression in taste buds Cell and Tissue Research (2021) 385:557–570 This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s00441-021-03452-5
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Koyanagi_Eriko_isk_620 other

Comments_Koyanagi_Eriko_isk_620 other

Diss_小栁_江梨子_ISK620_2021 fulltext

Abstract _小栁_江梨子_4516810291_2021 abstract