1,5-アンヒドロ-D-フラクトースはミトコンドリアの生成を介して、In vitroでのロテノンによる神経細胞の損傷を防ぐ

• Material Type: 博士論文
• Title: 1,5-アンヒドロ-D-フラクトースはミトコンドリアの生成を介して、In vitroでのロテノンによる神経細胞の損傷を防ぐ
• Author/Editor: 笠毛, 友揮
• Author Heading: 笠毛, 友揮 カサモ, ユウキ
• Publication, Distribution, etc.: MDPI
• Alternative Title: 1,5-Anhydro-D-Fructose Protects against Rotenone-Induced Neuronal Damage In Vitro Through Mitochondrial Biogenesis
• Degree grantor/type: 鹿児島大学
• Date Granted: 2022-02-17
• Dissertation Number: 甲総研第631号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: 1,5-AF; 1,5-AG; metformin; Parkinson’s disease; parkinsonism; AMPK; PGC-1α; mitochondria; mitochondrial biogenesis; rotenone
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Mitochondrial functional abnormalities or quantitative decreases are considered to be one of the most plausible pathogenic mechanisms of Parkinson’s disease (PD). Thus, mitochondrial complex inhibitors are often used for the development of experimental PD. In this study, we used rotenone to create in vitro cell models of PD, then used these models to investigate the effects of 1,5-anhydro-D-fructose (1,5-AF), a monosaccharide with protective effects against a range of cytotoxic substances. Subsequently, we investigated the possible mechanisms of these protective effects in PC12 cells. The protection of 1,5-AF against rotenone-induced cytotoxicity was confirmed by increased cell viability and longer dendritic lengths in PC12 and primary neuronal cells. Furthermore, in rotenone-treated PC12 cells, 1,5-AF upregulated peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α) expression and enhanced its deacetylation, while increasing AMP-activated protein kinase (AMPK) phosphorylation. 1,5-AF treatment also increased mitochondrial activity in these cells. Moreover, PGC-1α silencing inhibited the cytoprotective and mitochondrial biogenic effects of 1,5-AF in PC12 cells. Therefore, 1,5-AF may activate PGC-1α through AMPK activation, thus leading to mitochondrial biogenic and cytoprotective effects. Together, our results suggest that 1,5-AF has therapeutic potential for development as a treatment for PD. Yuki Kasamo, Kiyoshi Kikuchi, Munekazu Yamakuchi, Shotaro Otsuka, Seiya Takada, Yuki Kambe, Takashi Ito, Ko-ichi Kawahara, Kazunori Arita, Koji Yoshimoto and Ikuro Maruyama 1,5-Anhydro-D-fructose Protects against Rotenone-Induced Neuronal Damage In Vitro through Mitochondrial Biogenesis International Journal of Molecular Sciences 2021, 22, 9941 https://doi.org/10.3390/ijms22189941
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Kasamo_Yuki_isk_631 other

Comments_Kasamo_Yuki_isk_631 other

Diss_笠毛_友揮_ISK631_2021 fulltext

Abstract_笠毛_友揮_4513810063_2021 abstract